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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [4]
- Immunohistochemistry [1]
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- Product number
- LS-C185341 - Provider product page
- Provider
- LSBio
- Product name
- DDB1 Antibody (aa749-1140) LS-C185341
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. DDB1 antibody diluted at 1:1000.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. DDB1 antibody diluted at 1:1000.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. DDB1 antibody diluted at 1:5000.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed HeLa using DDB1 antibody at 1:200 dilution.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed HeLa using DDB1 antibody at 1:200 dilution.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 ug of DDB1 antibody 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 ug of DDB1 antibody 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 ug of DDB1 antibody 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 ug of DDB1 antibody 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Cal27 Xenograft using DDB1 antibody at 1:500 dilution.
