GTX100129

antibody from GeneTex

Targeting: DDB1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX100129

Provider

GeneTex

Proper citation

GeneTex Cat#GTX100129, RRID:AB_1240693

Product name

DDB1 antibody [N1N3]

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [N1N3] (GTX100129) diluted at 1:5000.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

Antibody Lot Number

39384

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX100129 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 £gg of whole cell lysate)A:HeLa S3 (GTX14654)B:Raji(GTX27908)7.5% SDS PAGEGTX100129 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [N1N3] (GTX100129) diluted at 1:5000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

DDB1 antibody [N1N3] detects DDB1 protein at nucleus and cytoplasm by immunofluorescent analysis.Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DDB1 protein stained by DDB1 antibody [N1N3] (GTX100129) diluted at 1:500.Blue: Hoechst 33342 staining.