PA5-34631

antibody from Invitrogen Antibodies

Targeting: DDB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34631

Provider

Invitrogen Antibodies

Product name

DDB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, A549, H1299, HCT116, MCF-7, Neuro2A, GL261, C8D30, PC-12.

Concentration

1 mg/mL

References

Submitted references

A novel DDB2 mutation causes defective recognition of UV-induced DNA damages and prevalent equine squamous cell carcinoma.

Chen L, Bellone RR, Wang Y, Singer-Berk M, Sugasawa K, Ford JM, Artandi SE
DNA repair 2021 Jan;97:103022

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDB1 was performed by separating 30 µg of PC-12 lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a DDB1 Polyclonal Antibody (Product # PA5-34631) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDB1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a DDB1 Polyclonal Antibody (Product # PA5-34631) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A: Neuro2A, B: GL261.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDB1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDSPAGE. Proteins were transferred to a membrane and probed with a DDB1 Polyclonal Antibody (Product # PA5-34631) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A: A549, B: H1299, C: HCT116, D: MCF-7.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of DDB1 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: DDB1 Polyclonal Antibody (Product # PA5-34631) diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of DDB1 was performed in paraffin-embedded U373 xenograft tissue using DDB1 Polyclonal Antibody (Product # PA5-34631) at a dilution of 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of DDB1 was performed in paraffin-embedded mouse duodenum tissue using DDB1 Polyclonal Antibody (Product # PA5-34631) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of DDB1 was performed in paraffin-embedded mouse hind brain tissue using DDB1 Polyclonal Antibody (Product # PA5-34631) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of DDB1 was performed in paraffin-embedded mouse heart tissue using DDB1 Polyclonal Antibody (Product # PA5-34631) at a dilution of 1:500.