FNab02285

antibody from FineTest Biotech

Targeting: DDB1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

FNab02285

Provider

FineTest Biotech

Product name

DDB1 antibody

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex(the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway(the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers(CPD), 6-4 photoproducts(6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX(DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2)(also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8)(the CSA complex) plays a role in transcription-coupled repair(TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

damage-specific DNA binding protein
1, 127kDa


Epitope

IgG2a

Isotype

IgG

Antibody clone number

3A0

Vial size

100¦Ìg

Concentration

Protein A+G purification

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)

Handling

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)