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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-21282 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HepG2, NIH-3T3, mouse brain, PC-12, Rat2, rat brain. Predicted reactivity: Mouse (99%), Rat (100%), Xenopus laevis (96%), Xenopus Tropicalis (93%), Chicken (98%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.81 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDB1 in paraformaldehyde-fixed HeLa cells using a DDB1 polyclonal antibody (Product # PA5-21282) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDB1 antibody (Product # PA5-21282) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDB1 Polyclonal Antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 µg of DDB1 Polyclonal Antibody (Product # PA5-21282) 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 Polyclonal Antibody (Product # PA5-21282) diluted at 1:1,000.
