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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-27113 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLaS3, Raji, mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDB1 Polyclonal Antibody (Product # PA5-27113). Sample (30 µg of whole cell lysate). A: HeLa S3. B: Raji. 7.5% SDS PAGE. DDB1 Polyclonal Antibody (Product # PA5-27113) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDB1 Polyclonal Antibody (Product # PA5-27113). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. DDB1 Polyclonal Antibody (Product # PA5-27113) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDB1 showing staining in the nucleus and cytoplasm of A375 cells. A375 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a DDB1 polyclonal antibody (Product # PA5-27113) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDB1 Polyclonal Antibody [N1N3] detects DDB1 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDB1 protein stained by DDB1 Polyclonal Antibody [N1N3] (Product # PA5-27113) diluted at 1:500. Blue: Hoechst 33342 staining.
