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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29990 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHI3L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431.
- Concentration
- 0.62 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CHI3L2 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CHI3L2 Polyclonal Antibody (Product # PA5-29990) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CHI3L2 Polyclonal Antibody (Product # PA5-29990). Sample (30 µg of whole cell lysate). Lane A: A431. 10% SDS PAGE. CHI3L2 Polyclonal Antibody . CHI3L2 Polyclonal Antibody (Product # PA5-29990) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CHI3L2 antibody detects CHI3L2 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. CHI3L2 antibody (Product # PA5-29990) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
