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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00010265-M06 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010265-M06, RRID:AB_530096
- Product name
- IRX5 monoclonal antibody (M06), clone 8A12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant IRX5.
- Antigen sequence
PQKPEDKADPEGPEAGGAEQKAASGCERLQGPPTP
AGKETEGSLSDSDFKEPPSEGRLDAL- Isotype
- IgG
- Antibody clone number
- 8A12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IRX5 monoclonal antibody (M06), clone 8A12. Western Blot analysis of IRX5 expression in Raw 264.7.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IRX5 monoclonal antibody (M06), clone 8A12. Western Blot analysis of IRX5 expression in NIH/3T3(Cat # L018V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged IRX5 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to IRX5 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
