Antibody data
- Antibody Data
 - Antigen structure
 - References [1]
 - Comments [0]
 - Validations
 - Western blot [2]
 - ELISA [1]
 - Immunocytochemistry [1]
 
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Validation data
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- Product number
 - H00010265-M06 - Provider product page

 - Provider
 - Novus Biologicals
 - Proper citation
 - Novus Cat#H00010265-M06, RRID:AB_2126077
 - Product name
 - Mouse Monoclonal Iroquois-class homeodomain protein IRX-5 Antibody
 - Antibody type
 - Monoclonal
 - Description
 - IgG purified. IRX5 - iroquois homeobox protein 5
 - Reactivity
 - Human, Mouse
 - Host
 - Mouse
 - Isotype
 - IgG
 - Vial size
 - 0.1 mg
 - Storage
 - Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
 
Submitted references		ADAR1 forms a complex with Dicer to promote microRNA processing and RNA-induced gene silencing.
				
		
	
			Ota H, Sakurai M, Gupta R, Valente L, Wulff BE, Ariyoshi K, Iizasa H, Davuluri RV, Nishikura K
Cell 2013 Apr 25;153(3):575-89
		Cell 2013 Apr 25;153(3):575-89
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					Supportive validation
					
									
				
				- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Western Blot: Iroquois-class homeodomain protein IRX-5 Antibody (8A12) [H00010265-M06] - Analysis of IRX5 expression in NIH/3T3 (Cat # L018V1).
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Western Blot: Iroquois-class homeodomain protein IRX-5 Antibody (8A12) [H00010265-M06] - Analysis of IRX5 expression in Raw 264.7.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - ELISA: Iroquois-class homeodomain protein IRX-5 Antibody (8A12) [H00010265-M06] - Detection limit for recombinant GST tagged IRX5 is approximately 0.3ng/ml as a capture antibody.
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunocytochemistry/Immunofluorescence: Iroquois-class homeodomain protein IRX-5 Antibody (8A12) [H00010265-M06] - Analysis of monoclonal antibody to IRX5 on HeLa cell. Antibody concentration 10 ug/ml