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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB8224 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TCF7/TCF1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse TCF7/TCF1 in ELISA and Western Blot.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q00417- Isotype
- IgG
- Antibody clone number
- 812145
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse TCF7/TCF1 by Western Blot. Western blot shows lysates of EL-4 mouse lymphoblast cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TCF7/TCF1 at approximately 30-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TCF7/TCF1 in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
