MA5-14972

antibody from Invitrogen Antibodies

Targeting: TCF7 TCF-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-14972

Provider

Invitrogen Antibodies

Product name

TCF7 Monoclonal Antibody (G.452.9)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with LEF1.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

G.452.9

Vial size

100 µL

Concentration

163 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TCF1 in total cell lysates from HCT29 and Colo201 cells using TCF1 monoclonal antibody (Product # MA5-14972).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using TCF7 Monoclonal Antibody (G.452.9) (Product # MA5-14972) and bands at 30 kDa and 50 kDa corresponding to TCF7 were observed in MOLT4, Jurkat, SW480 and Mouse Thymus; and was absent in U2OS, LnCAP, Mouse skeletal Muscle and Mouse Kidney. Whole cell extracts (30 µg lysate) of MOLT4 (Lane 1), Jurkat (Lane 2), SW480 (Lane 3), U2OS (Lane 4), LnCAP (Lane 5), tissue extracts (30 µg lysate) of Mouse Thymus (Lane 6), Mouse Kidney (Lane 7) and Mouse Skeletal Muscle (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of TCF7 was performed using 70% confluent log phase MOLT-4 and LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TCF7 Monoclonal Antibody (3D3) (Product # MA5-14972) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to nucleus. Panel e shows LNCaP cells with no expression of TCF7. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.