Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-54289 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MURC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KGKDRTVAEG EECAREMGVD IIARSESLGP ISELYSDELS EPEHEAARPV YPPHEGREIP TPEPLKVTFK SQVKVEDDES LLL Highest antigen sequence identity to the following orthologs: Mouse - 64%, Rat - 59%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of MURC in control (vector only transfected HEK293T lysate) and MURC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a MURC Polyclonal Antibody (Product # PA5-54289).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MURC in human cell line RH-30 using MURC Polyclonal Antibody (Product # PA5-54289) shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MURC in human cerebral cortex using MURC Polyclonal Antibody (Product # PA5-54289).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MURC in human skeletal muscle shows distinct cytoplasmic and membranous positivity in myocytes. Samples were probed using a MURC Polyclonal Antibody (Product # PA5-54289).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MURC in human colon using MURC Polyclonal Antibody (Product # PA5-54289).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MURC in human skeletal muscle and pancreas tissues using MURC Polyclonal Antibody (Product # PA5-54289). Corresponding MURC RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MURC in human cerebral cortex, colon, pancreas and skeletal muscle using MURC Polyclonal Antibody (Product # PA5-54289) (A) shows similar protein distribution across tissues to an independent MURC Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MURC in human pancreas using MURC Polyclonal Antibody (Product # PA5-54289) shows low expression as expected.