NBP2-15135
antibody from Novus Biologicals
Targeting: TET1
bA119F7.1, CXXC6, KIAA1676, LCX
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- NBP2-15135 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TET1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Differential Regulation of Methylation-Regulating Enzymes by Senescent Stromal Cells Drives Colorectal Cancer Cell Response to DNA-Demethylating Epi-Drugs.
Agrawal K, Das V, Táborská N, Gurský J, Džubák P, Hajdúch M
Stem cells international 2018;2018:6013728
Stem cells international 2018;2018:6013728
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TET1 Antibody (GT1462) [NBP2-15135] - A. 50 ug whole cell lysate/extract from 293T cells transfected with scramble siRNA . B. 50 ug whole cell lysate/extract from TET1-knockdowned 293T cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TET1 Antibody (1462) [NBP2-15135] - NT2D1 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [1462] diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TET1 Antibody (1462) [NBP2-15135] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells 5 % SDS-PAGE TET1 antibody dilution: 1:500
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TET1 Antibody (1462) [NBP2-15135] - Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 ug of TET1 antibody [1462] . Western blot analysis was performed using TET1 antibody [1462] . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135] - Detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin- Embedded HeLa xenograft. Dilution: 1:100