Explore
Validate
LearnGTX124207
antibody from GeneTex
Targeting: TET1
bA119F7.1, CXXC6, KIAA1676, LCX
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Western blot [8]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX124207 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX124207, RRID:AB_11176491
- Product name
- TET1 antibody [N3C1]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
Submitted references Abnormal activity of transcription factors gli in high-grade gliomas.
LIN28A activates the transcription of NANOG in dairy goat male germline stem cells.
High Dietary Fat Intake Affects DNA Methylation/Hydroxymethylation in Mouse Heart: Epigenetic Hints for Obesity-Related Cardiac Dysfunction.
Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Ginsenoside 20(S)-Rg3 Inhibits the Warburg Effect Via Modulating DNMT3A/ MiR-532-3p/HK2 Pathway in Ovarian Cancer Cells.
α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis.
TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells.
Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
IDH1 or -2 mutations do not predict outcome and do not cause loss of 5-hydroxymethylcytosine or altered histone modifications in central chondrosarcomas.
p53 is essential for DNA methylation homeostasis in naïve embryonic stem cells, and its loss promotes clonal heterogeneity.
Association of TET1 expression with colorectal cancer progression.
Genome-Wide DNA Methylation Variations upon Exposure to Engineered Nanomaterials and Their Implications in Nanosafety Assessment.
Hepatic cyclooxygenase-2 overexpression induced spontaneous hepatocellular carcinoma formation in mice.
Hypoxia-induced TET1 facilitates trophoblast cell migration and invasion through HIF1α signaling pathway.
5-Hydroxymethylcytosine discriminates between parathyroid adenoma and carcinoma.
Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum.
FOXA1 potentiates lineage-specific enhancer activation through modulating TET1 expression and function.
ERK/c-Jun Recruits Tet1 to Induce Zta Expression and Epstein-Barr Virus Reactivation through DNA Demethylation.
Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism.
TET enzymes are successively expressed during human spermatogenesis and their expression level is pivotal for male fertility.
Altering TET dioxygenase levels within physiological range affects DNA methylation dynamics of HEK293 cells.
5mC-hydroxylase activity is influenced by the PARylation of TET1 enzyme.
Aberrant TET1 Methylation Closely Associated with CpG Island Methylator Phenotype in Colorectal Cancer.
Poly(ADP-ribosyl)ation is involved in the epigenetic control of TET1 gene transcription.
TET1 is a maintenance DNA demethylase that prevents methylation spreading in differentiated cells.
Persistently altered epigenetic marks in the mouse uterus after neonatal estrogen exposure.
Upregulation of TET1 and downregulation of APOBEC3A and APOBEC3C in the parietal cortex of psychotic patients.
TET1 suppresses cancer invasion by activating the tissue inhibitors of metalloproteinases.
Volnitskiy A, Shtam T, Burdakov V, Kovalev R, Konev A, Filatov M
PloS one 2019;14(2):e0211980
PloS one 2019;14(2):e0211980
LIN28A activates the transcription of NANOG in dairy goat male germline stem cells.
Ma F, Du X, Wei Y, Zhou Z, Clotaire DZJ, Li N, Peng S, Li G, Hua J
Journal of cellular physiology 2019 Jun;234(6):8113-8121
Journal of cellular physiology 2019 Jun;234(6):8113-8121
High Dietary Fat Intake Affects DNA Methylation/Hydroxymethylation in Mouse Heart: Epigenetic Hints for Obesity-Related Cardiac Dysfunction.
Ciccarone F, Castelli S, Ioannilli L, Ciriolo MR
Molecular nutrition & food research 2019 Feb;63(4):e1800970
Molecular nutrition & food research 2019 Feb;63(4):e1800970
Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Pei YF, Xu XN, Wang ZF, Wang FW, Wu WD, Geng JF, Liu XQ
The American journal of pathology 2019 Feb 5;
The American journal of pathology 2019 Feb 5;
Ginsenoside 20(S)-Rg3 Inhibits the Warburg Effect Via Modulating DNMT3A/ MiR-532-3p/HK2 Pathway in Ovarian Cancer Cells.
Zhou Y, Zheng X, Lu J, Chen W, Li X, Zhao L
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(6):2548-2559
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(6):2548-2559
α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis.
Atlante S, Visintin A, Marini E, Savoia M, Dianzani C, Giorgis M, Sürün D, Maione F, Schnütgen F, Farsetti A, Zeiher AM, Bertinaria M, Giraudo E, Spallotta F, Cencioni C, Gaetano C
Cell death & disease 2018 Jul 9;9(7):756
Cell death & disease 2018 Jul 9;9(7):756
TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells.
Fan J, Zhang Y, Mu J, He X, Shao B, Zhou D, Peng W, Tang J, Jiang Y, Ren G, Xiang T
Clinical epigenetics 2018 Aug 3;10(1):103
Clinical epigenetics 2018 Aug 3;10(1):103
Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, Araúzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Schöler HR
Cell stem cell 2018 Aug 2;23(2):266-275.e6
Cell stem cell 2018 Aug 2;23(2):266-275.e6
IDH1 or -2 mutations do not predict outcome and do not cause loss of 5-hydroxymethylcytosine or altered histone modifications in central chondrosarcomas.
Cleven AHG, Suijker J, Agrogiannis G, Briaire-de Bruijn IH, Frizzell N, Hoekstra AS, Wijers-Koster PM, Cleton-Jansen AM, Bovée JVMG
Clinical sarcoma research 2017;7:8
Clinical sarcoma research 2017;7:8
p53 is essential for DNA methylation homeostasis in naïve embryonic stem cells, and its loss promotes clonal heterogeneity.
Tovy A, Spiro A, McCarthy R, Shipony Z, Aylon Y, Allton K, Ainbinder E, Furth N, Tanay A, Barton M, Oren M
Genes & development 2017 May 15;31(10):959-972
Genes & development 2017 May 15;31(10):959-972
Association of TET1 expression with colorectal cancer progression.
Tian Y, Pan F, Sun X, Gan M, Lin A, Zhang D, Zhu Y, Lai M
Scandinavian journal of gastroenterology 2017 Mar;52(3):312-320
Scandinavian journal of gastroenterology 2017 Mar;52(3):312-320
Genome-Wide DNA Methylation Variations upon Exposure to Engineered Nanomaterials and Their Implications in Nanosafety Assessment.
Chen Y, Xu M, Zhang J, Ma J, Gao M, Zhang Z, Xu Y, Liu S
Advanced materials (Deerfield Beach, Fla.) 2017 Feb;29(6)
Advanced materials (Deerfield Beach, Fla.) 2017 Feb;29(6)
Hepatic cyclooxygenase-2 overexpression induced spontaneous hepatocellular carcinoma formation in mice.
Chen H, Cai W, Chu ESH, Tang J, Wong CC, Wong SH, Sun W, Liang Q, Fang J, Sun Z, Yu J
Oncogene 2017 Aug;36(31):4415-4426
Oncogene 2017 Aug;36(31):4415-4426
Hypoxia-induced TET1 facilitates trophoblast cell migration and invasion through HIF1α signaling pathway.
Zhu J, Wang K, Li T, Chen J, Xie D, Chang X, Yao J, Wu J, Zhou Q, Jia Y, Duan T
Scientific reports 2017 Aug 14;7(1):8077
Scientific reports 2017 Aug 14;7(1):8077
5-Hydroxymethylcytosine discriminates between parathyroid adenoma and carcinoma.
Barazeghi E, Gill AJ, Sidhu S, Norlén O, Dina R, Palazzo FF, Hellman P, Stålberg P, Westin G
Clinical epigenetics 2016;8:31
Clinical epigenetics 2016;8:31
Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum.
Zhou FC, Resendiz M, Lo CL, Chen Y
PloS one 2016;11(9):e0162063
PloS one 2016;11(9):e0162063
FOXA1 potentiates lineage-specific enhancer activation through modulating TET1 expression and function.
Yang YA, Zhao JC, Fong KW, Kim J, Li S, Song C, Song B, Zheng B, He C, Yu J
Nucleic acids research 2016 Sep 30;44(17):8153-64
Nucleic acids research 2016 Sep 30;44(17):8153-64
ERK/c-Jun Recruits Tet1 to Induce Zta Expression and Epstein-Barr Virus Reactivation through DNA Demethylation.
Zhang W, Han D, Wan P, Pan P, Cao Y, Liu Y, Wu K, Wu J
Scientific reports 2016 Oct 6;6:34543
Scientific reports 2016 Oct 6;6:34543
Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism.
Weber AR, Krawczyk C, Robertson AB, Kuśnierczyk A, Vågbø CB, Schuermann D, Klungland A, Schär P
Nature communications 2016 Mar 2;7:10806
Nature communications 2016 Mar 2;7:10806
TET enzymes are successively expressed during human spermatogenesis and their expression level is pivotal for male fertility.
Ni K, Dansranjavin T, Rogenhofer N, Oeztuerk N, Deuker J, Bergmann M, Schuppe HC, Wagenlehner F, Weidner W, Steger K, Schagdarsurengin U
Human reproduction (Oxford, England) 2016 Jul;31(7):1411-24
Human reproduction (Oxford, England) 2016 Jul;31(7):1411-24
Altering TET dioxygenase levels within physiological range affects DNA methylation dynamics of HEK293 cells.
Grosser C, Wagner N, Grothaus K, Horsthemke B
Epigenetics 2015;10(9):819-33
Epigenetics 2015;10(9):819-33
5mC-hydroxylase activity is influenced by the PARylation of TET1 enzyme.
Ciccarone F, Valentini E, Zampieri M, Caiafa P
Oncotarget 2015 Sep 15;6(27):24333-47
Oncotarget 2015 Sep 15;6(27):24333-47
Aberrant TET1 Methylation Closely Associated with CpG Island Methylator Phenotype in Colorectal Cancer.
Ichimura N, Shinjo K, An B, Shimizu Y, Yamao K, Ohka F, Katsushima K, Hatanaka A, Tojo M, Yamamoto E, Suzuki H, Ueda M, Kondo Y
Cancer prevention research (Philadelphia, Pa.) 2015 Aug;8(8):702-11
Cancer prevention research (Philadelphia, Pa.) 2015 Aug;8(8):702-11
Poly(ADP-ribosyl)ation is involved in the epigenetic control of TET1 gene transcription.
Ciccarone F, Valentini E, Bacalini MG, Zampieri M, Calabrese R, Guastafierro T, Mariano G, Reale A, Franceschi C, Caiafa P
Oncotarget 2014 Nov 15;5(21):10356-67
Oncotarget 2014 Nov 15;5(21):10356-67
TET1 is a maintenance DNA demethylase that prevents methylation spreading in differentiated cells.
Jin C, Lu Y, Jelinek J, Liang S, Estecio MR, Barton MC, Issa JP
Nucleic acids research 2014 Jun;42(11):6956-71
Nucleic acids research 2014 Jun;42(11):6956-71
Persistently altered epigenetic marks in the mouse uterus after neonatal estrogen exposure.
Jefferson WN, Chevalier DM, Phelps JY, Cantor AM, Padilla-Banks E, Newbold RR, Archer TK, Kinyamu HK, Williams CJ
Molecular endocrinology (Baltimore, Md.) 2013 Oct;27(10):1666-77
Molecular endocrinology (Baltimore, Md.) 2013 Oct;27(10):1666-77
Upregulation of TET1 and downregulation of APOBEC3A and APOBEC3C in the parietal cortex of psychotic patients.
Dong E, Gavin DP, Chen Y, Davis J
Translational psychiatry 2012 Sep 4;2:e159
Translational psychiatry 2012 Sep 4;2:e159
TET1 suppresses cancer invasion by activating the tissue inhibitors of metalloproteinases.
Hsu CH, Peng KL, Kang ML, Chen YR, Yang YC, Tsai CH, Chu CS, Jeng YM, Chen YT, Lin FM, Huang HD, Lu YY, Teng YC, Lin ST, Lin RK, Tang FM, Lee SB, Hsu HM, Yu JC, Hsiao PW, Juan LJ
Cell reports 2012 Sep 27;2(3):568-79
Cell reports 2012 Sep 27;2(3):568-79
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- (A) western blot analysis of endogenous TET1 expression in HeLa (lane 1) and HepG2 cell (lane 2). (B) 293T cell are mock transfected (lane1 and 3) or with tagged-hTET1 (lane 2 and 4) for 24hrs, using either GTX124207 (diluted at 1:10000) (left) or anti-tag (right) antibody. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TET1 antibody [N3C1] detects TET1 protein by Western blot analysis.A. 30 µg HeLa whole cell lysate/extractB. 30 µg HepG2 whole cell lysate/extract5 % SDS-PAGETET1 antibody [N3C1] (GTX124207) dilution: 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TET1 antibody [N3C1] detects TET1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells5% SDS-PAGETET1 antibody [N3C1] (GTX124207) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis. Sample: Paraffin-embedded Human normal prostate tissue. TET1 antibody [N3C1] (GTX124207) dilution: 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (GTX124207) antibody at 1:1000 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . TET1 antibody [N3C1] (GTX124207) diluted at 1:250.
