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- Immunocytochemistry [1]
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- Product number
- PA5-72805 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TET1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Sarcosine Suppresses Epileptogenesis in Rats With Effects on Hippocampal DNA Methylation.
Shen HY, Weltha L, Cook JM, Gesese R, Omi W, Baer SB, Rose RM, Reemmer J, Boison D
Frontiers in molecular neuroscience 2020;13:97
Frontiers in molecular neuroscience 2020;13:97
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of TET1 in mouse brain tissue with TET1 Polyclonal Antibody (Product # PA5-72805) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TET1 in human brain tissue with TET1 Polyclonal Antibody (Product # PA5-72805) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TET1 in mouse brain tissue with TET1 Polyclonal Antibody (Product # PA5-72805) at 20 µg/mL. Red: TET1 Blue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TET1 in human brain tissue with TET1 Polyclonal Antibody (Product # PA5-72805) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 Changes of DNA methylation in kindling epileptogenesis. (A) Immunofluorescence staining for 5mC and 5hmC n the hippocampus of fully kindled rats vs. non-kindled naive controls. Scale bar = 250 mum (a-f) and 25 mum (g,h). Quantitative analysis of the DG levels of (B) 5mC and (C) 5hmC in non-kindled naive controls and fully kindled rats. (D) Representative Western blot imaging of TET1, DNMT1, and DNMT3a. (E) Quantification of hippocampal levels of TET1, DNMT1, and DNMT3a in fully kindled rats vs. non-kindled naive controls. Data are mean +- SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. non-kindled naive controls.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 Sarcosine affects DNA methylase and demethylase. (A) Representative Western blot imaging of TET1, DNMT1, and DNMT3a of the hippocampus of rats exposed to sarcosine during kindling and dissected on experimental days 6 and 11. (B) Quantification of hippocampal levels of TET1, DNMT1, and DNMT3a in sarcosine-treated rats vs. vehicle-treated controls. Data are mean +- SEM. ** p < 0.01 vs. controls.
