H00005578-M01

antibody from Novus Biologicals

Targeting: PRKCA PKCA, PKCα

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

H00005578-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00005578-M01, RRID:AB_547075

Product name

Mouse Monoclonal PKC alpha Antibody

Antibody type

Monoclonal

Description

IgG purified. PRKCA - protein kinase C, alpha

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PKC alpha Antibody (2F11) [H00005578-M01] - PRKCA monoclonal antibody (M01), clone 2F11 Analysis of PRKCA expression in HeLa.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: PKC alpha Antibody (2F11) [H00005578-M01] - Detection limit for recombinant GST tagged PRKCA is approximately 0.1ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (2F11) [H00005578-M01] - Analysis of monoclonal antibody to PRKCA on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PKC alpha Antibody (2F11) [H00005578-M01] - Analysis of monoclonal antibody to PRKCA on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.