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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4531 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4531, RRID:AB_1579128
- Product name
- PRKCA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRKCA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Protein kinase C-alpha phosphorylation of specific serines in the connecting segment of the beta 4 integrin regulates the dynamics of type II hemidesmosomes.
Lactosylceramide recruits PKCalpha/epsilon and phospholipase A2 to stimulate PECAM-1 expression in human monocytes and adhesion to endothelial cells.
Cell volume regulation in response to hypotonicity is impaired in HeLa cells expressing a protein kinase Calpha mutant lacking kinase activity.
Rabinovitz I, Tsomo L, Mercurio AM
Molecular and cellular biology 2004 May;24(10):4351-60
Molecular and cellular biology 2004 May;24(10):4351-60
Lactosylceramide recruits PKCalpha/epsilon and phospholipase A2 to stimulate PECAM-1 expression in human monocytes and adhesion to endothelial cells.
Gong N, Wei H, Chowdhury SH, Chatterjee S
Proceedings of the National Academy of Sciences of the United States of America 2004 Apr 27;101(17):6490-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Apr 27;101(17):6490-5
Cell volume regulation in response to hypotonicity is impaired in HeLa cells expressing a protein kinase Calpha mutant lacking kinase activity.
Hermoso M, Olivero P, Torres R, Riveros A, Quest AF, Stutzin A
The Journal of biological chemistry 2004 Apr 23;279(17):17681-9
The Journal of biological chemistry 2004 Apr 23;279(17):17681-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKCA polyclonal antibody (Cat # PAB4531) in placenta lysate . PRKCA (arrow) was detected using purified PRKCA polyclonal antibody (Cat # PAB4531) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCA polyclonal antibody (Cat # PAB4531) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
