PA5-22160

antibody from Invitrogen Antibodies

Targeting: PRKCA PKCA, PKCα

Antibody data

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Validation data

Product number: PA5-22160 - Provider product page
Provider: Invitrogen Antibodies
Product name: PKC alpha Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: H1299, Jurkat, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (89%), Xenopus laevis (92%), Rabbit (99%), Chicken (95%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.69 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PRKCA protein structure - PA5-22160 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKC using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC polyclonal antibody (Product # PA5-22160) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PRKCA in whole cell extract (30 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with PRKCA Polyclonal antibody (Product # PA5-22160) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PKC alpha was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC alpha Polyclonal Antibody (Product # PA5-22160) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PKC alpha was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC alpha Polyclonal Antibody (Product # PA5-22160) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PKC alpha was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC alpha Polyclonal Antibody (Product # PA5-22160) at a dilution of 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PKC alpha Polyclonal Antibody (Product # PA5-22160). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCA antibody PKC alpha Polyclonal Antibody (Product # PA5-22160) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PKC alpha Polyclonal Antibody (Product # PA5-22160). Sample (30 µg of whole cell lysate). Lane A: H1299 . 7.5% SDS PAGE. PKC alpha Polyclonal Antibody (Product # PA5-22160) diluted at 1:1,000.