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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-29228 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKC alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Mouse brain. Predicted reactivity: Mouse (100%), Zebrafish (90%), Xenopus laevis (94%), Rabbit (100%), Chicken (98%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.74 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKC alpha using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC alpha polyclonal antibody (Product # PA5-29228) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PKC alpha was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC alpha Polyclonal Antibody (Product # PA5-29228) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PKC alpha Polyclonal Antibody (Product # PA5-29228). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. PKC alpha Polyclonal Antibody (Product # PA5-29228) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PKC alpha (Product # PA5-29228) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
