21991-1-AP

antibody from Proteintech Group

Targeting: PRKCA PKCA, PKCα

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

21991-1-AP

Provider

Proteintech Group

Product name

PKC Alpha antibody

Antibody type

Polyclonal

Description

KD/KO validated PKC Alpha antibody (Cat. #21991-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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RSK2-mediated phosphorylation and degradation of UBE2O inhibits hepatocellular carcinoma growth and resistance to radiotherapy.

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Human iPSC-derived retinal organoids develop robust Alzheimer's disease neuropathology.

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Omics-based pharmacological evaluation reveals Yuanhu Zhitong oral liquid ameliorates arthritis by regulating PKC/ERK/NF-κB signaling pathway.

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The resistance to anoikis, mediated by Spp1, and the evasion of immune surveillance facilitate the invasion and metastasis of hepatocellular carcinoma.

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Proteomics reveals the pharmacological mechanism of flavonoids from Astragali Complanati Semen in preventing chronic liver injury.

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Super-Enhancer-Driven Syndecan-4 Regulates Intercellular Communication in Hypoxic Pulmonary Hypertension.

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Sphincter of Oddi Dysfunction Induces Gallstone by Inhibiting the Expression of ABCB11 via PKC-α.

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Oryza sativa L. Indica Seed Coat Ameliorated Concanavalin A-Induced Acute Hepatitis in Mice via MDM2/p53 and PKCα/MAPK1 Signaling Pathways.

Zhao Z, Li Y, Guo S, Chen Y, Yin H, Li Y, Cheng G, Tian L
International journal of molecular sciences 2023 Sep 25;24(19)

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Transient receptor potential vanilloid 4 (TRPV4) in neutrophils enhances myocardial ischemia/reperfusion injury.

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Network Analysis and Experimental Verification of the Mechanisms of Hydroxysafflor Yellow A in Ischemic Stroke Following Atherosclerosis.

Han X, Zhou H, Yin J, Zhu J, Yang J, Wan H
Molecules (Basel, Switzerland) 2023 Nov 28;28(23)

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Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter.

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bioRxiv : the preprint server for biology 2023 Nov 21;

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MAPK4 predicts poor prognosis and facilitates the proliferation and migration of glioma through the AKT/mTOR pathway.

Ren J, Zheng S, Zhang L, Liu J, Cao H, Wu S, Xu Y, Sun J
Cancer medicine 2023 May;12(10):11624-11640

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Selenomethionine promotes ANXA2 phosphorylation for proliferation and protein synthesis of myoblasts and skeletal muscle growth.

Zhang M, Li X, Cui X, Li R, Ma Z, Gao X
The Journal of nutritional biochemistry 2023 May;115:109277

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Lead-induced cardiomyocytes apoptosis by inhibiting gap junction intercellular communication via modulating the PKCα/Cx43 signaling pathway.

Wang Q, Ma Y, Li Y, He Z, Feng B
Chemico-biological interactions 2023 May 1;376:110451

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SLC7A14 imports GABA to lysosomes and impairs hepatic insulin sensitivity via inhibiting mTORC2.

Jiang X, Liu K, Jiang H, Yin H, Wang ED, Cheng H, Yuan F, Xiao F, Wang F, Lu W, Peng B, Shu Y, Li X, Chen S, Guo F
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Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia.

Gao S, Chen Z, Shi J, Chen Z, Yun D, Li X, Wu X, Sun F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Aug;37(8):e23081

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Gluconolactone Alleviates Myocardial Ischemia/Reperfusion Injury and Arrhythmias via Activating PKCε/Extracellular Signal-Regulated Kinase Signaling.

Qin X, Liu B, Gao F, Hu Y, Chen Z, Xu J, Zhang X
Frontiers in physiology 2022;13:856699

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Cellular mechanism of action of forsythiaside for the treatment of diabetic kidney disease.

Xu C, Miao H, Chen X, Zhang H
Frontiers in pharmacology 2022;13:1096536

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miR-381-3p Cooperated With Hes1 to Regulate the Proliferation and Differentiation of Retinal Progenitor Cells.

Wang J, Sun N, Ju Y, Ni N, Tang Z, Zhang D, Dai X, Chen M, Wang Y, Gu P, Ji J
Frontiers in cell and developmental biology 2022;10:853215

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PKC signal amplification suppresses non-small cell lung cancer growth by promoting p21 expression and phosphorylation.

Liu S, Zhang Y, Yang Q, Zhang Y, Liu H, Huang MH, Wang R, Lu F
Heliyon 2022 Sep;8(9):e10657

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Porcine circovirus-like virus P1 activates pancreatic secretion pathway by interacting with CHRM3 protein.

Zhu J, Xiao Q, Yin L, Zhang F, Wen L, Suolang S, He K
Veterinary microbiology 2022 Sep;272:109495

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Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth.

Gu C, Zhang W, Yang E, Gu C, Zhang Z, Ke J, Wang X, Wu S, Li S, Wu F
Oncogenesis 2022 Sep 15;11(1):55

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GPRC6A is a key mediator of palmitic acid regulation of lipid synthesis in bovine mammary epithelial cells.

Jin X, Zhen Z, Wang Z, Gao X, Li M
Cell biology international 2022 Nov;46(11):1747-1758

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Ameliorative effect of Gastrodia elata Blume extracts on depression in zebrafish and cellular models through modulating reticulon 4 receptors and apoptosis.

Wang R, Ren Q, Gao D, Paudel YN, Li X, Wang L, Zhang P, Wang B, Shang X, Jin M
Journal of ethnopharmacology 2022 May 10;289:115018

---

ATF4/CEMIP/PKCα promotes anoikis resistance by enhancing protective autophagy in prostate cancer cells.

Yu Y, Liu B, Li X, Lu D, Yang L, Chen L, Li Y, Cheng L, Lv F, Zhang P, Song Y, Xing Y
Cell death & disease 2022 Jan 10;13(1):46

---

Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.

Zhang P, He Q, Wang Y, Zhou G, Chen Y, Tang L, Zhang Y, Hong X, Mao Y, He Q, Yang X, Liu N, Ma J
Signal transduction and targeted therapy 2022 Feb 16;7(1):46

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PEBP4 Directs the Malignant Behavior of Hepatocellular Carcinoma Cells via Regulating mTORC1 and mTORC2.

Chen Q, Jin J, Guo W, Tang Z, Luo Y, Ying Y, Lin H, Luo Z
International journal of molecular sciences 2022 Aug 8;23(15)

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Proteomic Analysis of Emodin Treatment in Neuropathic Pain Reveals Dysfunction of the Calcium Signaling Pathway.

Chen P, Lin D, Wang C, Song C, Wang W, Qu J, Wu Z
Journal of pain research 2021;14:613-622

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Dephosphorylation of annexin A2 by protein phosphatase 1 regulates endothelial cell barrier.

Király N, Thalwieser Z, Fonódi M, Csortos C, Boratkó A
IUBMB life 2021 Oct;73(10):1257-1268

---

LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin.

Su X, Wei J, Qi H, Jin M, Zhang Q, Zhang Y, Zhang C, Yang R
Inflammatory bowel diseases 2021 Jul 27;27(8):1302-1315

---

β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.

Lu Y, Li X, Liu H, Xue J, Zeng Z, Dong X, Zhang T, Wu G, Yang K, Xu S
Oncogene 2021 Feb;40(7):1269-1283

---

Small molecule screening identified cepharanthine as an inhibitor of porcine reproductive and respiratory syndrome virus infection in vitro by suppressing integrins/ILK/RACK1/PKCα/NF-κB signalling axis.

Yang C, Zuo Q, Liu X, Zhao Q, Pu H, Gao L, Zhao L, Guo Z, Lin Y, Liu J, Bi J, Yin G
Veterinary microbiology 2021 Apr;255:109016

---

MED13L integrates Mediator-regulated epigenetic control into lung cancer radiosensitivity.

Zhang N, Song Y, Xu Y, Liu J, Shen Y, Zhou L, Yu J, Yang M
Theranostics 2020;10(20):9378-9394

---

Inhibition of porcine reproductive and respiratory syndrome virus by PKC inhibitor dequalinium chloride in vitro.

Liu X, Gao L, Zhao Q, Wang X, Yang C, Bi J, Yang R, Jin X, Lan R, Cui R, Wang X, Li W, Wang X, Yang Y, Yu X, Lin Y, Liu J, Yin G
Veterinary microbiology 2020 Dec;251:108913

---

Reduced expression of annexin A1 promotes gemcitabine and 5-fluorouracil drug resistance of human pancreatic cancer.

Liu QH, Yong HM, Zhuang QX, Zhang XP, Hou PF, Chen YS, Zhu MH, Bai J
Investigational new drugs 2020 Apr;38(2):350-359

---

Novel synthetic tosyl chloride-berbamine regresses lethal MYC-positive leukemia by targeting CaMKIIγ/Myc axis.

Yu Q, Wang P, Yang L, Wu Z, Li S, Xu Y, Wu B, Ma A, Gan X, Xu R
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Sep;117:109134

---

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Wang X, Adegoke EO, Ma M, Huang F, Zhang H, Adeniran SO, Zheng P, Zhang G
Theriogenology 2019 Oct 15;138:84-93

---

HDGF and PRKCA upregulation is associated with a poor prognosis in patients with lung adenocarcinoma.

Jiang H, Fu Q, Song X, Ge C, Li R, Li Z, Zeng B, Li C, Wang Y, Xue Y, Luo R, Fang W
Oncology letters 2019 Nov;18(5):4936-4946

---

Silencing of Transcription Factor Sp1 Promotes SN1 Transporter Regulation by Ammonia in Mouse Cortical Astrocytes.

Dąbrowska K, Zielińska M
International journal of molecular sciences 2019 Jan 9;20(2)

---

Differential effects of N-acetylcysteine on retinal degeneration in two mouse models of normal tension glaucoma.

Sano H, Namekata K, Kimura A, Shitara H, Guo X, Harada C, Mitamura Y, Harada T
Cell death & disease 2019 Jan 28;10(2):75

---

Phosphorylation of ELAVL1 (Ser219/Ser316) mediated by PKC is required for erythropoiesis.

Zhou X, Wang S, Zheng M, Kuver A, Wan X, Dai K, Li X
Biochimica et biophysica acta. Molecular cell research 2019 Feb;1866(2):214-224

---

Sensory plasticity of carotid body is correlated with oxidative stress in paraventricular nucleus during chronic intermittent hypoxia.

Liu P, Zhang HM, Hu K, Zhou XF, Tang S
Journal of cellular physiology 2019 Aug;234(8):13534-13543

---

Silencing Formin-like 2 inhibits growth and metastasis of gastric cancer cells through suppressing internalization of integrins.

Zhong B, Wang K, Xu H, Kong F
Cancer cell international 2018;18:79

---

Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes.

Dąbrowska K, Albrecht J, Zielińska M
Neurochemistry international 2018 Sep;118:225-232

---

β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.

Ma J, Lu Y, Zhang S, Li Y, Huang J, Yin Z, Ren J, Huang K, Liu L, Yang K, Wu G, Xu S
Cell death and differentiation 2018 Aug;25(8):1473-1485

---

Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.

Akaiwa K, Namekata K, Azuchi Y, Sano H, Guo X, Kimura A, Harada C, Mitamura Y, Harada T
Investigative ophthalmology & visual science 2018 Apr 1;59(5):2080-2089

---

Dioscin Inhibits HSC-T6 Cell Migration via Adjusting SDC-4 Expression: Insights from iTRAQ-Based Quantitative Proteomics.

Yin L, Qi Y, Xu Y, Xu L, Han X, Tao X, Song S, Peng J
Frontiers in pharmacology 2017;8:665

---

A comparative proteomics study on matrix vesicles of osteoblast-like Saos-2 and U2-OS cells.

Jiang L, Cui Y, Luan J, Zhou X, Zhou X, Han J
Intractable & rare diseases research 2013 May;2(2):59-62

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The PKC alpha antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human PKC alpha. This antibody recognizes human, mouse antigen. The PKC alpha antibody has been validated for the following applications: ELISA, WB analysis.

Appendix

21991-1-AP.pdf

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