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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14053 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14053, RRID:AB_10676427
- Product name
- PADI4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PADI4.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 ug) with PADI4 polyclonal antibody (Cat # PAB14053), diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of PADI4 polyclonal antibody (Cat # PAB14053) in antigen coated wells.By plotting the absorbance against the crude serum dilution, the titer of the antibody was estimated to be 1 : 40,200.
- Validation comment
- ELISA
