Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-22317 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PADI4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PAD4-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.44 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Neutrophil Extracellular Traps Promote Angiogenesis: Evidence From Vascular Pathology in Pulmonary Hypertension.
Lymphocryptovirus Infection of Nonhuman Primate B Cells Converts Destructive into Productive Processing of the Pathogenic CD8 T Cell Epitope in Myelin Oligodendrocyte Glycoprotein.
Aldabbous L, Abdul-Salam V, McKinnon T, Duluc L, Pepke-Zaba J, Southwood M, Ainscough AJ, Hadinnapola C, Wilkins MR, Toshner M, Wojciak-Stothard B
Arteriosclerosis, thrombosis, and vascular biology 2016 Oct;36(10):2078-87
Arteriosclerosis, thrombosis, and vascular biology 2016 Oct;36(10):2078-87
Lymphocryptovirus Infection of Nonhuman Primate B Cells Converts Destructive into Productive Processing of the Pathogenic CD8 T Cell Epitope in Myelin Oligodendrocyte Glycoprotein.
Jagessar SA, Holtman IR, Hofman S, Morandi E, Heijmans N, Laman JD, Gran B, Faber BW, van Kasteren SI, Eggen BJ, 't Hart BA
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1074-88
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1074-88
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PAD4 in paraformaldehyde-fixed HeLa cells using a PAD4 polyclonal antibody (Product # PA5-22317) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PA5-22317 antibody immunoprecipitates PAD4 protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract with 5 µg preimmune rabbit IgG (A) or 5 µg of PAD4 antibody (Product # PA5-22317) (B). The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL