Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [13]
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Validation data
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- Product number
- NBP1-87353 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87353, RRID:AB_11035677
- Product name
- Rabbit Polyclonal PADI4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PADI4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: PADI4 Antibody [NBP1-87353] - Analysis in control (vector only transfected HEK293T lysate) and pADI4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow shows strong nuclear positivity in poietic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human kidney shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human spleen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Analysis in human bone marrow and kidney tissues. Corresponding PADI4 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody NBP1-87353 (A) shows similar protein distribution across tissues to independent antibody NBP2-38499 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human kidney shows no nuclear positivity as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human spleen shows strong nuclear positivity in cells in red pulp.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human testis shows no nuclear positivity in cells in seminiferous ducts as expected.