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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-61600 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61600, RRID:AB_963527
- Product name
- Rabbit Polyclonal pre-mRNA cleavage factor I (59 kDa subunit) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Canine, Guinea Pig, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Global quantitative proteomics reveal up-regulation of endoplasmic reticulum stress response proteins upon depletion of eIF5A in HeLa cells.
Temporal association of herpes simplex virus ICP4 with cellular complexes functioning at multiple steps in PolII transcription.
Mandal A, Mandal S, Park MH
Scientific reports 2016 May 16;6:25795
Scientific reports 2016 May 16;6:25795
Temporal association of herpes simplex virus ICP4 with cellular complexes functioning at multiple steps in PolII transcription.
Wagner LM, DeLuca NA
PloS one 2013;8(10):e78242
PloS one 2013;8(10):e78242
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CPSF59 antibody NB100-61600 used for IP at 3 mcg/mg lysate. CPSF59 was also immunoprecipitated by rabbit anti-CPSF59 antibody NB100-61599, which recognizes an upstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - ICP4 containing complexes include TFIIH, cleavage and polyadenylation complex and chromatin remodeling complexes. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against CPSF2 and CPSF7 of the cleavage and polyadenylation complex were used. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-CPSF59 used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61600] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CPSF59 used at a dilution of 1:500 (2ug/ml). Detection: DAB
