Antibody data
- Antibody Data
- Antigen structure
- References [102]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Other assay [5]
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Validation data
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- Product number
- MA5-13156 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin Pan Type I/II Antibody Cocktail
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-13156 targets Cytokeratin Pan in WB, IF and IHC (P) applications and shows reactivity with Bovine, Chicken, Human, mouse, Non-human primate, Rabbit, and Rat samples. This antibody is not suitable for PC12 cells in IF or WB applications.
- Antibody clone number
- AE1/AE3
- Concentration
- 0.2 mg/mL
Submitted references Spatial collagen stiffening promotes collective breast cancer cell invasion by reinforcing extracellular matrix alignment.
The prognostic impact of the tumour stroma fraction: A machine learning-based analysis in 16 human solid tumour types.
A novel method for isolation and culture of primary swine gastric epithelial cells.
Stromal SOX2 Upregulation Promotes Tumorigenesis through the Generation of a SFRP1/2-Expressing Cancer-Associated Fibroblast Population.
Topographical Distribution and Spatial Interactions of Innate and Semi-Innate Immune Cells in Pancreatic and Other Periampullary Adenocarcinoma.
EPS8 phosphorylation by Src modulates its oncogenic functions.
Prognostic Interactions between FAP+ Fibroblasts and CD8a+ T Cells in Colon Cancer.
Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology.
Quantitative, qualitative and spatial analysis of lymphocyte infiltration in periampullary and pancreatic adenocarcinoma.
Polymorphonuclear MDSCs are enriched in the stroma and expanded in metastases of prostate cancer.
Cell swelling, softening and invasion in a three-dimensional breast cancer model.
Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer.
Age-associated functional morphology of thyroid and its impact on the expression of vimentin, cytokeratins and VEGF. The role of nigella in refinement.
Macrophages induce "budding" in aggressive human colon cancer subtypes by protease-mediated disruption of tight junctions.
The morphological regeneration and functional restoration of bladder defects by a novel scaffold and adipose-derived stem cells in a rat augmentation model.
Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro.
A Previously Undescribed Presentation of Mixed Adenoneuroendocrine Carcinoma.
Human breast cancer biopsies induce eosinophil recruitment and enhance adjacent cancer cell proliferation.
Doxorubicin and cisplatin induce apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue.
Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793).
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
Fumarate induces redox-dependent senescence by modifying glutathione metabolism.
The carboxy-terminus of p63 links cell cycle control and the proliferative potential of epidermal progenitor cells.
Investigation of the origin of stromal and endothelial cells at the desmoplastic interface in xenograft tumor in mice.
The first microbial environment of infants born by C-section: the operating room microbes.
Establishment and characterization of fin-derived cell line from ornamental carp, Cyprinus carpio koi, for virus isolation in India.
Keratinocyte growth factor-2 and autologous serum potentiate the regenerative effect of mesenchymal stem cells in cornea damage in rats.
Superficial soft tissue biphasic synovial sarcoma with apocrine differentiation in the glandular component: a report of two cases.
Liquid-based cytologic findings of solitary extramedullary plasmacytoma in thyroid: a case report identified with fine-needle aspiration cytology.
Invasive thymoma metastatic to the cavernous sinus.
Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma.
Correlation between the area of high-signal intensity on SPIO-enhanced MR imaging and the pathologic size of sentinel node metastases in breast cancer patients with positive sentinel nodes.
T-Cell tropism of simian varicella virus during primary infection.
Poor-prognosis colon cancer is defined by a molecularly distinct subtype and develops from serrated precursor lesions.
Malignant ossifying fibromyxoid tumor of the tongue: case report and review of the literature.
Epstein-Barr virus transcytosis through polarized oral epithelial cells.
The effects of hyperbaric oxygen application against cholestatic oxidative stress and hepatic damage after bile duct ligation in rats.
Is mammary not otherwise specified-type sarcoma with CD10 expression a distinct entity? A rare case report with immunohistochemical and ultrastructural study.
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells.
Sentinel nodes identified by computed tomography-lymphography accurately stage the axilla in patients with breast cancer.
Epidermal adrenergic signaling contributes to inflammation and pain sensitization in a rat model of complex regional pain syndrome.
Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Dural angioleiomyoma of the middle cranial fossa: a case report and review of the literature.
Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.
Localization of estrogen receptor in the central lymphoid organs of chickens during the late stage of embryogenesis.
Validation of three breast cancer nomograms and a new formula for predicting non-sentinel lymph node status.
Smooth muscle tension induces invasive remodeling of the zebrafish intestine.
Rgs19 regulates mouse palatal fusion by modulating cell proliferation and apoptosis in the MEE.
A novel function for p21Cip1 and acetyltransferase p/CAF as critical transcriptional regulators of TGFβ-mediated breast cancer cell migration and invasion.
A novel TP53 somatic mutation involved in the pathogenesis of pediatric choroid plexus carcinoma.
Low-temperature electrospun silk scaffold for in vitro mucosal modeling.
FTIR microscopy of biological cells and tissue: data analysis using resonant Mie scattering (RMieS) EMSC algorithm.
Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
Giant angiofibromas in tuberous sclerosis complex: a possible role for localized lymphedema in their pathogenesis.
Substance P signaling controls mast cell activation, degranulation, and nociceptive sensitization in a rat fracture model of complex regional pain syndrome.
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Congenital stridor in the context of Chiari malformation type II: the etiological role of vernix caseosa granulomatous meningitis.
Distinct histological features characterize primary angiosarcoma of bone.
Morphological evidences in circumvallate papilla and von Ebners' gland development in mice.
Intra-uterine tissue engineering of full-thickness skin defects in a fetal sheep model.
Ventilator-induced inflammatory response in lipopolysaccharide-exposed rat lung is mediated by angiotensin-converting enzyme.
Antiproliferative and apoptotic effects of the herbal agent Pygeum africanum on cultured prostate stromal cells from patients with benign prostatic hyperplasia (BPH).
Exacerbated innate host response to SARS-CoV in aged non-human primates.
Fracture induces keratinocyte activation, proliferation, and expression of pro-nociceptive inflammatory mediators.
The clinical significance of tumor cells in bone marrow or apheresis product and the efficacy of CD34+ selection and high-dose chemotherapy in patients with Stage III breast cancer.
Epidermotropic chondroid metastasis of melanoma: report of a case of metastatic melanoma with previously unreported morphological features.
Rhabdomyosarcoma arising in a mature cystic teratoma with contralateral serous carcinoma: case report and review of the literature.
Metastasis of adenocarcinoma of the gall bladder to an endometrial polyp detected by endometrial curettage: case report and review of the literature.
Colony-stimulating factor-1-dependent macrophage functions regulate the maternal decidua immune responses against Listeria monocytogenes infections during early gestation in mice.
Multifocal metanephric adenoma in childhood.
Primary choroid plexus papilloma of the sacral nerve roots.
Giant cell tumor-like lesion of the urinary bladder: a report of two cases and literature review; giant cell tumor or undifferentiated carcinoma?
The NALP1 inflammasome controls cytokine production and nociception in a rat fracture model of complex regional pain syndrome.
Carcinoid-like pattern in melanoma: report of 4 cases.
Post-junctional facilitation of Substance P signaling in a tibia fracture rat model of complex regional pain syndrome type I.
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
Deletion of the ectodomain unleashes the transforming, invasive, and tumorigenic potential of the MET oncogene.
Plasmoblastic lymphoma associated with human immunodeficiency virus.
The coexistence of invasive ductal carcinoma and malignant phyllodes tumor with liposarcomatous and chondrosarcomatous differentiation in the same breast in a post-osteosarcoma case.
Targeting a single mismatched minor histocompatibility antigen with tumor-restricted expression eradicates human solid tumors.
Epithelial and pseudoepithelial differentiation in glioblastoma and gliosarcoma: a comparative morphologic and molecular genetic study.
Adenomatoid tumour of the liver.
Clinocopathologic and immunohistochemical findings of multiple genital leiomyomas and mammary adenocarcinomas in a bitch.
Eccrine syringofibroadenoma associated with well-differentiated squamous cell carcinoma.
Chromophobe renal cell carcinoma with osseous metaplasia containing fatty bone marrow element: a case report.
Myointimoma of the glans penis.
Detection of micrometastases and skip metastases with ex vivo sentinel node mapping in carcinoma of the colon and rectum.
Re-evaluation of axillary skip metastases in the era of sentinel lymph node biopsy in breast cancer.
Malignant perivascular epithelioid cell tumor of the colon: report of a case with molecular analysis.
Atypical reactive ovarian surface epithelium, a pitfall in pathologic assessment.
Identification of major cell types in paraffin sections of bovine tissues.
Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.
Cyclooxygenase-2 overexpression in MCF-10F human breast epithelial cells inhibits proliferation, apoptosis and differentiation, and causes partial transformation.
Pigmented solid-pseudopapillary neoplasm of the pancreas.
Epithelioid angiomyolipoma of the kidney.
Sarcomatoid carcinoma of the renal pelvis with giant cell tumor-like features: case report with immunohistochemical findings.
The absence of delayed graft function is predicted by the presence of manganese-superoxide dismutase in distal tubules of renal allografts.
Adrenocortical oncocytic tumors: report of 10 cases and review of the literature.
Oncocytic adrenocortical carcinomas: a pathological and immunohistochemical study of four cases in comparison with conventional adrenocortical carcinomas.
Ectopic thyroid in the gallbladder: report of a case.
Automated acquisition of stained tissue microarrays for high-throughput evaluation of molecular targets.
Invasion of interstitial matrix by a novel cell line from primary peritoneal carcinosarcoma, and by established ovarian carcinoma cell lines: role of cell-matrix adhesion molecules, proteinases, and E-cadherin expression.
Koorman T, Jansen KA, Khalil A, Haughton PD, Visser D, Rätze MAK, Haakma WE, Sakalauskaitè G, van Diest PJ, de Rooij J, Derksen PWB
Oncogene 2022 Apr;41(17):2458-2469
Oncogene 2022 Apr;41(17):2458-2469
The prognostic impact of the tumour stroma fraction: A machine learning-based analysis in 16 human solid tumour types.
Micke P, Strell C, Mattsson J, Martín-Bernabé A, Brunnström H, Huvila J, Sund M, Wärnberg F, Ponten F, Glimelius B, Hrynchyk I, Mauchanski S, Khelashvili S, Garcia-Vicién G, Molleví DG, Edqvist PH, O Reilly A, Corvigno S, Dahlstrand H, Botling J, Segersten U, Krzyzanowska A, Bjartell A, Elebro J, Heby M, Lundgren S, Hedner C, Borg D, Brändstedt J, Sartor H, Malmström PU, Johansson M, Nodin B, Backman M, Lindskog C, Jirström K, Mezheyeuski A
EBioMedicine 2021 Mar;65:103269
EBioMedicine 2021 Mar;65:103269
A novel method for isolation and culture of primary swine gastric epithelial cells.
Bautista-Amorocho H, Silva-Sayago JA, Goyeneche-Patino DA, Pérez-Cala TL, Macías-Gómez F, Arango-Viana JC, Martínez A
BMC molecular and cell biology 2021 Jan 6;22(1):1
BMC molecular and cell biology 2021 Jan 6;22(1):1
Stromal SOX2 Upregulation Promotes Tumorigenesis through the Generation of a SFRP1/2-Expressing Cancer-Associated Fibroblast Population.
Kasashima H, Duran A, Martinez-Ordoñez A, Nakanishi Y, Kinoshita H, Linares JF, Reina-Campos M, Kudo Y, L'Hermitte A, Yashiro M, Ohira M, Bao F, Tauriello DVF, Batlle E, Diaz-Meco MT, Moscat J
Developmental cell 2021 Jan 11;56(1):95-110.e10
Developmental cell 2021 Jan 11;56(1):95-110.e10
Topographical Distribution and Spatial Interactions of Innate and Semi-Innate Immune Cells in Pancreatic and Other Periampullary Adenocarcinoma.
Lundgren S, Micke P, Elebro J, Heby M, Hrynchyk I, Nodin B, Leandersson K, Mezheyeuski A, Jirström K
Frontiers in immunology 2020;11:558169
Frontiers in immunology 2020;11:558169
EPS8 phosphorylation by Src modulates its oncogenic functions.
Shahoumi LA, Khodadadi H, Bensreti H, Baban B, Yeudall WA
British journal of cancer 2020 Sep;123(7):1078-1088
British journal of cancer 2020 Sep;123(7):1078-1088
Prognostic Interactions between FAP+ Fibroblasts and CD8a+ T Cells in Colon Cancer.
Herrera M, Mezheyeuski A, Villabona L, Corvigno S, Strell C, Klein C, Hölzlwimmer G, Glimelius B, Masucci G, Sjöblom T, Östman A
Cancers 2020 Nov 3;12(11)
Cancers 2020 Nov 3;12(11)
Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology.
Bucur O, Fu F, Calderon M, Mylvaganam GH, Ly NL, Day J, Watkin S, Walker BD, Boyden ES, Zhao Y
Nature protocols 2020 May;15(5):1649-1672
Nature protocols 2020 May;15(5):1649-1672
Quantitative, qualitative and spatial analysis of lymphocyte infiltration in periampullary and pancreatic adenocarcinoma.
Lundgren S, Elebro J, Heby M, Nodin B, Leandersson K, Micke P, Jirström K, Mezheyeuski A
International journal of cancer 2020 Jun 15;146(12):3461-3473
International journal of cancer 2020 Jun 15;146(12):3461-3473
Polymorphonuclear MDSCs are enriched in the stroma and expanded in metastases of prostate cancer.
Wen J, Huang G, Liu S, Wan J, Wang X, Zhu Y, Kaliney W, Zhang C, Cheng L, Wen X, Lu X
The journal of pathology. Clinical research 2020 Jul;6(3):171-177
The journal of pathology. Clinical research 2020 Jul;6(3):171-177
Cell swelling, softening and invasion in a three-dimensional breast cancer model.
Han YL, Pegoraro AF, Li H, Li K, Yuan Y, Xu G, Gu Z, Sun J, Hao Y, Gupta SK, Li Y, Tang W, Tang X, Teng L, Fredberg JJ, Guo M
Nature physics 2020 Jan;16(1):101-108
Nature physics 2020 Jan;16(1):101-108
Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer.
Bhattacharyya S, Oon C, Kothari A, Horton W, Link J, Sears RC, Sherman MH
The Journal of experimental medicine 2020 Aug 3;217(8)
The Journal of experimental medicine 2020 Aug 3;217(8)
Age-associated functional morphology of thyroid and its impact on the expression of vimentin, cytokeratins and VEGF. The role of nigella in refinement.
Zaki SM, Mohamed EA, Abdel Fattah S, Abdullah H, Kaszubowska L
Folia histochemica et cytobiologica 2018;56(3):159-171
Folia histochemica et cytobiologica 2018;56(3):159-171
Macrophages induce "budding" in aggressive human colon cancer subtypes by protease-mediated disruption of tight junctions.
Trumpi K, Frenkel N, Peters T, Korthagen NM, Jongen JMJ, Raats D, van Grevenstein H, Backes Y, Moons LM, Lacle MM, Koster J, Zwijnenburg D, Borel Rinkes IHM, Kranenburg O
Oncotarget 2018 Apr 13;9(28):19490-19507
Oncotarget 2018 Apr 13;9(28):19490-19507
The morphological regeneration and functional restoration of bladder defects by a novel scaffold and adipose-derived stem cells in a rat augmentation model.
Wang Q, Xiao DD, Yan H, Zhao Y, Fu S, Zhou J, Wang Z, Zhou Z, Zhang M, Lu MJ
Stem cell research & therapy 2017 Jun 24;8(1):149
Stem cell research & therapy 2017 Jun 24;8(1):149
Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro.
Wu W, Wu XL, Ji YQ, Gao Z
Molecular medicine reports 2017 Jul;16(1):95-100
Molecular medicine reports 2017 Jul;16(1):95-100
A Previously Undescribed Presentation of Mixed Adenoneuroendocrine Carcinoma.
De Luca-Johnson J, Zenali M
Case reports in pathology 2016;2016:9063634
Case reports in pathology 2016;2016:9063634
Human breast cancer biopsies induce eosinophil recruitment and enhance adjacent cancer cell proliferation.
Szalayova G, Ogrodnik A, Spencer B, Wade J, Bunn J, Ambaye A, James T, Rincon M
Breast cancer research and treatment 2016 Jun;157(3):461-74
Breast cancer research and treatment 2016 Jun;157(3):461-74
Doxorubicin and cisplatin induce apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue.
Fabbri R, Macciocca M, Vicenti R, Paradisi R, Klinger FG, Pasquinelli G, Spisni E, Seracchioli R, Papi A
Future oncology (London, England) 2016 Jul;12(14):1699-711
Future oncology (London, England) 2016 Jul;12(14):1699-711
Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793).
Sood N, Chaudhary DK, Pradhan PK, Verma DK, Raja Swaminathan T, Kushwaha B, Punia P, Jena JK
In vitro cellular & developmental biology. Animal 2015 Sep;51(8):787-96
In vitro cellular & developmental biology. Animal 2015 Sep;51(8):787-96
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
Sauter JL, Ambaye AB, Mount SL
Diagnostic cytopathology 2015 Sep;43(9):688-95
Diagnostic cytopathology 2015 Sep;43(9):688-95
Fumarate induces redox-dependent senescence by modifying glutathione metabolism.
Zheng L, Cardaci S, Jerby L, MacKenzie ED, Sciacovelli M, Johnson TI, Gaude E, King A, Leach JD, Edrada-Ebel R, Hedley A, Morrice NA, Kalna G, Blyth K, Ruppin E, Frezza C, Gottlieb E
Nature communications 2015 Jan 23;6:6001
Nature communications 2015 Jan 23;6:6001
The carboxy-terminus of p63 links cell cycle control and the proliferative potential of epidermal progenitor cells.
Suzuki D, Sahu R, Leu NA, Senoo M
Development (Cambridge, England) 2015 Jan 15;142(2):282-90
Development (Cambridge, England) 2015 Jan 15;142(2):282-90
Investigation of the origin of stromal and endothelial cells at the desmoplastic interface in xenograft tumor in mice.
Jung M, Ryu YJ, Kang G
Pathology, research and practice 2015 Dec;211(12):925-30
Pathology, research and practice 2015 Dec;211(12):925-30
The first microbial environment of infants born by C-section: the operating room microbes.
Shin H, Pei Z, Martinez KA 2nd, Rivera-Vinas JI, Mendez K, Cavallin H, Dominguez-Bello MG
Microbiome 2015 Dec 1;3:59
Microbiome 2015 Dec 1;3:59
Establishment and characterization of fin-derived cell line from ornamental carp, Cyprinus carpio koi, for virus isolation in India.
Swaminathan TR, Basheer VS, Kumar R, Kathirvelpandian A, Sood N, Jena JK
In vitro cellular & developmental biology. Animal 2015 Aug;51(7):705-13
In vitro cellular & developmental biology. Animal 2015 Aug;51(7):705-13
Keratinocyte growth factor-2 and autologous serum potentiate the regenerative effect of mesenchymal stem cells in cornea damage in rats.
Pınarlı FA, Okten G, Beden U, Fışgın T, Kefeli M, Kara N, Duru F, Tomak L
International journal of ophthalmology 2014;7(2):211-9
International journal of ophthalmology 2014;7(2):211-9
Superficial soft tissue biphasic synovial sarcoma with apocrine differentiation in the glandular component: a report of two cases.
Shelekhova KV, Calonje E, Grossmann P, Kacerovska D, Koudela K Jr, Mirka H, Michal M, Kazakov DV
The American Journal of dermatopathology 2014 Oct;36(10):847-52
The American Journal of dermatopathology 2014 Oct;36(10):847-52
Liquid-based cytologic findings of solitary extramedullary plasmacytoma in thyroid: a case report identified with fine-needle aspiration cytology.
Lee CH, Jung YY, Chung YR, Ryu HS
Diagnostic cytopathology 2014 Nov;42(11):964-9
Diagnostic cytopathology 2014 Nov;42(11):964-9
Invasive thymoma metastatic to the cavernous sinus.
Nassiri F, Scheithauer BW, Corwin DJ, Kaplan HG, Mayberg M, Cusimano MD, Rotondo F, Kovacs K
Surgical neurology international 2013;4:74
Surgical neurology international 2013;4:74
Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma.
Qiu SQ, Wei XL, Huang WH, Wu MY, Qin YS, Li YK, Zhang GJ
Scientific reports 2013;3:2529
Scientific reports 2013;3:2529
Correlation between the area of high-signal intensity on SPIO-enhanced MR imaging and the pathologic size of sentinel node metastases in breast cancer patients with positive sentinel nodes.
Motomura K, Izumi T, Tateishi S, Sumino H, Noguchi A, Horinouchi T, Nakanishi K
BMC medical imaging 2013 Sep 13;13:32
BMC medical imaging 2013 Sep 13;13:32
T-Cell tropism of simian varicella virus during primary infection.
Ouwendijk WJ, Mahalingam R, de Swart RL, Haagmans BL, van Amerongen G, Getu S, Gilden D, Osterhaus AD, Verjans GM
PLoS pathogens 2013 May;9(5):e1003368
PLoS pathogens 2013 May;9(5):e1003368
Poor-prognosis colon cancer is defined by a molecularly distinct subtype and develops from serrated precursor lesions.
De Sousa E Melo F, Wang X, Jansen M, Fessler E, Trinh A, de Rooij LP, de Jong JH, de Boer OJ, van Leersum R, Bijlsma MF, Rodermond H, van der Heijden M, van Noesel CJ, Tuynman JB, Dekker E, Markowetz F, Medema JP, Vermeulen L
Nature medicine 2013 May;19(5):614-8
Nature medicine 2013 May;19(5):614-8
Malignant ossifying fibromyxoid tumor of the tongue: case report and review of the literature.
Ohta K, Taki M, Ogawa I, Ono S, Mizuta K, Fujimoto S, Takata T, Kamata N
Head & face medicine 2013 Jun 24;9:16
Head & face medicine 2013 Jun 24;9:16
Epstein-Barr virus transcytosis through polarized oral epithelial cells.
Tugizov SM, Herrera R, Palefsky JM
Journal of virology 2013 Jul;87(14):8179-94
Journal of virology 2013 Jul;87(14):8179-94
The effects of hyperbaric oxygen application against cholestatic oxidative stress and hepatic damage after bile duct ligation in rats.
Ayvaz S, Kanter M, Aksu B, Sahin SH, Uzun H, Erboga M, Pul M
The Journal of surgical research 2013 Jul;183(1):146-55
The Journal of surgical research 2013 Jul;183(1):146-55
Is mammary not otherwise specified-type sarcoma with CD10 expression a distinct entity? A rare case report with immunohistochemical and ultrastructural study.
Yang GZ, Li J, Jin H, Ding HY
Diagnostic pathology 2013 Jan 28;8:14
Diagnostic pathology 2013 Jan 28;8:14
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells.
Weli SC, Aamelfot M, Dale OB, Koppang EO, Falk K
Virology journal 2013 Jan 2;10:5
Virology journal 2013 Jan 2;10:5
Sentinel nodes identified by computed tomography-lymphography accurately stage the axilla in patients with breast cancer.
Motomura K, Sumino H, Noguchi A, Horinouchi T, Nakanishi K
BMC medical imaging 2013 Dec 9;13:42
BMC medical imaging 2013 Dec 9;13:42
Epidermal adrenergic signaling contributes to inflammation and pain sensitization in a rat model of complex regional pain syndrome.
Li W, Shi X, Wang L, Guo T, Wei T, Cheng K, Rice KC, Kingery WS, Clark JD
Pain 2013 Aug;154(8):1224-36
Pain 2013 Aug;154(8):1224-36
Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF
Cell stem cell 2013 Aug 1;13(2):246-54
Cell stem cell 2013 Aug 1;13(2):246-54
Dural angioleiomyoma of the middle cranial fossa: a case report and review of the literature.
Zhou Z, Yu M, Yang S, Zhou J, Sun R, Yang G
Brain tumor pathology 2013 Apr;30(2):117-21
Brain tumor pathology 2013 Apr;30(2):117-21
Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.
Marian C, Boila A, Soanca D, Malau M, Podeanu DM, Resetkova E, Stolnicu S
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2013 Apr;121(4):272-9
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2013 Apr;121(4):272-9
Localization of estrogen receptor in the central lymphoid organs of chickens during the late stage of embryogenesis.
Katayama M, Fukuda T, Narabara K, Abe A, Kondo Y
Bioscience, biotechnology, and biochemistry 2012;76(11):2003-7
Bioscience, biotechnology, and biochemistry 2012;76(11):2003-7
Validation of three breast cancer nomograms and a new formula for predicting non-sentinel lymph node status.
Derici S, Sevinc A, Harmancioglu O, Saydam S, Kocdor M, Aksoy S, Egeli T, Canda T, Ellidokuz H, Derici S
Asian Pacific journal of cancer prevention : APJCP 2012;13(12):6181-5
Asian Pacific journal of cancer prevention : APJCP 2012;13(12):6181-5
Smooth muscle tension induces invasive remodeling of the zebrafish intestine.
Seiler C, Davuluri G, Abrams J, Byfield FJ, Janmey PA, Pack M
PLoS biology 2012;10(9):e1001386
PLoS biology 2012;10(9):e1001386
Rgs19 regulates mouse palatal fusion by modulating cell proliferation and apoptosis in the MEE.
Sohn WJ, Ji YR, Kim HS, Gwon GJ, Chae YM, An CH, Park HD, Jung HS, Ryoo ZY, Lee S, Kim JY
Mechanisms of development 2012 Sep-Dec;129(9-12):244-54
Mechanisms of development 2012 Sep-Dec;129(9-12):244-54
A novel function for p21Cip1 and acetyltransferase p/CAF as critical transcriptional regulators of TGFβ-mediated breast cancer cell migration and invasion.
Dai M, Al-Odaini AA, Arakelian A, Rabbani SA, Ali S, Lebrun JJ
Breast cancer research : BCR 2012 Sep 20;14(5):R127
Breast cancer research : BCR 2012 Sep 20;14(5):R127
A novel TP53 somatic mutation involved in the pathogenesis of pediatric choroid plexus carcinoma.
Lv SQ, Song YC, Xu JP, Shu HF, Zhou Z, An N, Huang QL, Yang H
Medical science monitor : international medical journal of experimental and clinical research 2012 May;18(5):CS37-41
Medical science monitor : international medical journal of experimental and clinical research 2012 May;18(5):CS37-41
Low-temperature electrospun silk scaffold for in vitro mucosal modeling.
Bulysheva AA, Bowlin GL, Klingelhutz AJ, Yeudall WA
Journal of biomedical materials research. Part A 2012 Mar;100(3):757-67
Journal of biomedical materials research. Part A 2012 Mar;100(3):757-67
FTIR microscopy of biological cells and tissue: data analysis using resonant Mie scattering (RMieS) EMSC algorithm.
Bassan P, Sachdeva A, Kohler A, Hughes C, Henderson A, Boyle J, Shanks JH, Brown M, Clarke NW, Gardner P
The Analyst 2012 Mar 21;137(6):1370-7
The Analyst 2012 Mar 21;137(6):1370-7
Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
Wei T, Guo TZ, Li WW, Hou S, Kingery WS, Clark JD
Journal of neuroinflammation 2012 Jul 23;9:181
Journal of neuroinflammation 2012 Jul 23;9:181
Giant angiofibromas in tuberous sclerosis complex: a possible role for localized lymphedema in their pathogenesis.
Kacerovska D, Kerl K, Michal M, Filipova H, Vrtel R, Vanecek T, Zelenakova H, Kraus J, Kodet R, Kazakov DV
Journal of the American Academy of Dermatology 2012 Dec;67(6):1319-26
Journal of the American Academy of Dermatology 2012 Dec;67(6):1319-26
Substance P signaling controls mast cell activation, degranulation, and nociceptive sensitization in a rat fracture model of complex regional pain syndrome.
Li WW, Guo TZ, Liang DY, Sun Y, Kingery WS, Clark JD
Anesthesiology 2012 Apr;116(4):882-95
Anesthesiology 2012 Apr;116(4):882-95
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Yin WH, Li J, Chan JK
Diagnostic pathology 2012 Apr 12;7:39
Diagnostic pathology 2012 Apr 12;7:39
Congenital stridor in the context of Chiari malformation type II: the etiological role of vernix caseosa granulomatous meningitis.
Stritzke AI, Dunham CP, Smyth JA, Steinbok P
Journal of neurosurgery. Pediatrics 2011 Oct;8(4):372-6
Journal of neurosurgery. Pediatrics 2011 Oct;8(4):372-6
Distinct histological features characterize primary angiosarcoma of bone.
Verbeke SL, Bertoni F, Bacchini P, Sciot R, Fletcher CD, Kroon HM, Hogendoorn PC, Bovée JV
Histopathology 2011 Jan;58(2):254-64
Histopathology 2011 Jan;58(2):254-64
Morphological evidences in circumvallate papilla and von Ebners' gland development in mice.
Sohn WJ, Gwon GJ, An CH, Moon C, Bae YC, Yamamoto H, Lee S, Kim JY
Anatomy & cell biology 2011 Dec;44(4):274-83
Anatomy & cell biology 2011 Dec;44(4):274-83
Intra-uterine tissue engineering of full-thickness skin defects in a fetal sheep model.
Hosper NA, Eggink AJ, Roelofs LA, Wijnen RM, van Luyn MJ, Bank RA, Harmsen MC, Geutjes PJ, Daamen WF, van Kuppevelt TH, Tiemessen DM, Oosterwijk E, Crevels JJ, Blokx WA, Lotgering FK, van den Berg PP, Feitz WF
Biomaterials 2010 May;31(14):3910-9
Biomaterials 2010 May;31(14):3910-9
Ventilator-induced inflammatory response in lipopolysaccharide-exposed rat lung is mediated by angiotensin-converting enzyme.
Wösten-van Asperen RM, Lutter R, Specht PA, van Woensel JB, van der Loos CM, Florquin S, Lachmann B, Bos AP
The American journal of pathology 2010 May;176(5):2219-27
The American journal of pathology 2010 May;176(5):2219-27
Antiproliferative and apoptotic effects of the herbal agent Pygeum africanum on cultured prostate stromal cells from patients with benign prostatic hyperplasia (BPH).
Quiles MT, Arbós MA, Fraga A, de Torres IM, Reventós J, Morote J
The Prostate 2010 Jul 1;70(10):1044-53
The Prostate 2010 Jul 1;70(10):1044-53
Exacerbated innate host response to SARS-CoV in aged non-human primates.
Smits SL, de Lang A, van den Brand JM, Leijten LM, van IJcken WF, Eijkemans MJ, van Amerongen G, Kuiken T, Andeweg AC, Osterhaus AD, Haagmans BL
PLoS pathogens 2010 Feb 5;6(2):e1000756
PLoS pathogens 2010 Feb 5;6(2):e1000756
Fracture induces keratinocyte activation, proliferation, and expression of pro-nociceptive inflammatory mediators.
Li WW, Guo TZ, Li XQ, Kingery WS, Clark DJ
Pain 2010 Dec;151(3):843-852
Pain 2010 Dec;151(3):843-852
The clinical significance of tumor cells in bone marrow or apheresis product and the efficacy of CD34+ selection and high-dose chemotherapy in patients with Stage III breast cancer.
Arpaci F, Safali M, Ozet A, Ural AU, Cetin T, Ozturk M, Ataergin S, Kuzhan O, Komurcu S, Ozturk B, Acikel C
Journal of clinical apheresis 2009;24(5):197-204
Journal of clinical apheresis 2009;24(5):197-204
Epidermotropic chondroid metastasis of melanoma: report of a case of metastatic melanoma with previously unreported morphological features.
Piana S, Valli R, Ricci C
The American Journal of dermatopathology 2009 May;31(3):301-4
The American Journal of dermatopathology 2009 May;31(3):301-4
Rhabdomyosarcoma arising in a mature cystic teratoma with contralateral serous carcinoma: case report and review of the literature.
Kefeli M, Kandemir B, Akpolat I, Yildirim A, Kokcu A
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2009 Jul;28(4):372-5
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2009 Jul;28(4):372-5
Metastasis of adenocarcinoma of the gall bladder to an endometrial polyp detected by endometrial curettage: case report and review of the literature.
Kefeli M, Gonullu G, Can B, Malatyalioglu E, Kandemir B
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2009 Jul;28(4):343-6
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2009 Jul;28(4):343-6
Colony-stimulating factor-1-dependent macrophage functions regulate the maternal decidua immune responses against Listeria monocytogenes infections during early gestation in mice.
Qiu X, Zhu L, Pollard JW
Infection and immunity 2009 Jan;77(1):85-97
Infection and immunity 2009 Jan;77(1):85-97
Multifocal metanephric adenoma in childhood.
Kohashi K, Oda Y, Nakamori M, Yamamoto H, Tamiya S, Toubo T, Kinoshita Y, Tajiri T, Taguchi T, Tsuneyoshi M
Pathology international 2009 Jan;59(1):49-52
Pathology international 2009 Jan;59(1):49-52
Primary choroid plexus papilloma of the sacral nerve roots.
Boldorini R, Panzarasa G, Girardi P, Monga G
Journal of neurosurgery. Spine 2009 Jan;10(1):51-3
Journal of neurosurgery. Spine 2009 Jan;10(1):51-3
Giant cell tumor-like lesion of the urinary bladder: a report of two cases and literature review; giant cell tumor or undifferentiated carcinoma?
Behzatoğlu K, Durak H, Canberk S, Aydin O, Huq GE, Oznur M, Ozyalvaçli G, Yildiz P
Diagnostic pathology 2009 Dec 31;4:48
Diagnostic pathology 2009 Dec 31;4:48
The NALP1 inflammasome controls cytokine production and nociception in a rat fracture model of complex regional pain syndrome.
Li WW, Guo TZ, Liang D, Shi X, Wei T, Kingery WS, Clark JD
Pain 2009 Dec 15;147(1-3):277-86
Pain 2009 Dec 15;147(1-3):277-86
Carcinoid-like pattern in melanoma: report of 4 cases.
Kacerovska D, Michal M, Sosna B, Cempirkova D, Ambrus M, Richtr P, Danis D, Zelger B, Kazakov DV
The American Journal of dermatopathology 2009 Aug;31(6):542-50
The American Journal of dermatopathology 2009 Aug;31(6):542-50
Post-junctional facilitation of Substance P signaling in a tibia fracture rat model of complex regional pain syndrome type I.
Wei T, Li WW, Guo TZ, Zhao R, Wang L, Clark DJ, Oaklander AL, Schmelz M, Kingery WS
Pain 2009 Aug;144(3):278-286
Pain 2009 Aug;144(3):278-286
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
Mukhopadhyay S, El-Zammar OA, Katzenstein AL
The American journal of surgical pathology 2009 Apr;33(4):487-95
The American journal of surgical pathology 2009 Apr;33(4):487-95
Deletion of the ectodomain unleashes the transforming, invasive, and tumorigenic potential of the MET oncogene.
Merlin S, Pietronave S, Locarno D, Valente G, Follenzi A, Prat M
Cancer science 2009 Apr;100(4):633-8
Cancer science 2009 Apr;100(4):633-8
Plasmoblastic lymphoma associated with human immunodeficiency virus.
Horváth E, Krenács L, Bagdi E, Pávai Z, Macarie I, Nagy EE, Demian S
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2008;49(3):309-14
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2008;49(3):309-14
The coexistence of invasive ductal carcinoma and malignant phyllodes tumor with liposarcomatous and chondrosarcomatous differentiation in the same breast in a post-osteosarcoma case.
Kefeli M, Yildiz L, Akpolat I, Balci P, Ozen N
Pathology, research and practice 2008;204(12):919-23
Pathology, research and practice 2008;204(12):919-23
Targeting a single mismatched minor histocompatibility antigen with tumor-restricted expression eradicates human solid tumors.
Hambach L, Vermeij M, Buser A, Aghai Z, van der Kwast T, Goulmy E
Blood 2008 Sep 1;112(5):1844-52
Blood 2008 Sep 1;112(5):1844-52
Epithelial and pseudoepithelial differentiation in glioblastoma and gliosarcoma: a comparative morphologic and molecular genetic study.
Rodriguez FJ, Scheithauer BW, Giannini C, Bryant SC, Jenkins RB
Cancer 2008 Nov 15;113(10):2779-89
Cancer 2008 Nov 15;113(10):2779-89
Adenomatoid tumour of the liver.
Nagata S, Aishima S, Fukuzawa K, Takagi H, Yonemasu H, Iwashita Y, Kinoshita T, Wakasugi K
Journal of clinical pathology 2008 Jun;61(6):777-80
Journal of clinical pathology 2008 Jun;61(6):777-80
Clinocopathologic and immunohistochemical findings of multiple genital leiomyomas and mammary adenocarcinomas in a bitch.
Ozmen O, Haligur M, Kocamuftuoglu M
Reproduction in domestic animals = Zuchthygiene 2008 Jun;43(3):377-381
Reproduction in domestic animals = Zuchthygiene 2008 Jun;43(3):377-381
Eccrine syringofibroadenoma associated with well-differentiated squamous cell carcinoma.
Kacerovska D, Nemcova J, Michal M, Kazakov DV
The American Journal of dermatopathology 2008 Dec;30(6):572-4
The American Journal of dermatopathology 2008 Dec;30(6):572-4
Chromophobe renal cell carcinoma with osseous metaplasia containing fatty bone marrow element: a case report.
Kefeli M, Yildiz L, Aydin O, Kandemir B, Faik Yilmaz A
Pathology, research and practice 2007;203(10):749-52
Pathology, research and practice 2007;203(10):749-52
Myointimoma of the glans penis.
Vardar E, Gunlusoy B, Arslan M, Kececi S
Pathology international 2007 Mar;57(3):158-61
Pathology international 2007 Mar;57(3):158-61
Detection of micrometastases and skip metastases with ex vivo sentinel node mapping in carcinoma of the colon and rectum.
Yagci G, Unlu A, Kurt B, Can MF, Kaymakcioglu N, Cetiner S, Tufan T, Sen D
International journal of colorectal disease 2007 Feb;22(2):167-73
International journal of colorectal disease 2007 Feb;22(2):167-73
Re-evaluation of axillary skip metastases in the era of sentinel lymph node biopsy in breast cancer.
Keskek M, Balas S, Gokoz A, Sayek I
Surgery today 2006;36(12):1047-52
Surgery today 2006;36(12):1047-52
Malignant perivascular epithelioid cell tumor of the colon: report of a case with molecular analysis.
Yamamoto H, Oda Y, Yao T, Oiwa T, Kobayashi C, Tamiya S, Kawaguchi K, Hino O, Tsuneyoshi M
Pathology international 2006 Jan;56(1):46-50
Pathology international 2006 Jan;56(1):46-50
Atypical reactive ovarian surface epithelium, a pitfall in pathologic assessment.
Aydin NE, Celik O, Hasçalik S, Edali MN
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 2006 Jan-Feb;16 Suppl 1:207-10
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 2006 Jan-Feb;16 Suppl 1:207-10
Identification of major cell types in paraffin sections of bovine tissues.
Niku M, Ekman A, Pessa-Morikawa T, Iivanainen A
BMC veterinary research 2006 Jan 27;2:5
BMC veterinary research 2006 Jan 27;2:5
Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.
Yesim G, Gupse T, Zafer U, Ahmet A
Journal of hepato-biliary-pancreatic surgery 2005;12(6):502-7
Journal of hepato-biliary-pancreatic surgery 2005;12(6):502-7
Cyclooxygenase-2 overexpression in MCF-10F human breast epithelial cells inhibits proliferation, apoptosis and differentiation, and causes partial transformation.
Lu S, Yu G, Zhu Y, Archer MC
International journal of cancer 2005 Oct 10;116(6):847-52
International journal of cancer 2005 Oct 10;116(6):847-52
Pigmented solid-pseudopapillary neoplasm of the pancreas.
Daum O, Sima R, Mukensnabl P, Vanecek T, Brouckova M, Benes Z, Michal M
Pathology international 2005 May;55(5):280-4
Pathology international 2005 May;55(5):280-4
Epithelioid angiomyolipoma of the kidney.
Acikalin MF, Tel N, Oner U, Pasaoglu O, Dönmez T
International journal of urology : official journal of the Japanese Urological Association 2005 Feb;12(2):204-7
International journal of urology : official journal of the Japanese Urological Association 2005 Feb;12(2):204-7
Sarcomatoid carcinoma of the renal pelvis with giant cell tumor-like features: case report with immunohistochemical findings.
Acikalin MF, Kabukcuoglu S, Can C
International journal of urology : official journal of the Japanese Urological Association 2005 Feb;12(2):199-203
International journal of urology : official journal of the Japanese Urological Association 2005 Feb;12(2):199-203
The absence of delayed graft function is predicted by the presence of manganese-superoxide dismutase in distal tubules of renal allografts.
Boom H, de Heer E, van der Wal A, Kruidenier L, de Fijter JW, Benediktsson H, Paul LC, van Es LA
Transplantation 2005 Apr 27;79(8):946-52
Transplantation 2005 Apr 27;79(8):946-52
Adrenocortical oncocytic tumors: report of 10 cases and review of the literature.
Bisceglia M, Ludovico O, Di Mattia A, Ben-Dor D, Sandbank J, Pasquinelli G, Lau SK, Weiss LM
International journal of surgical pathology 2004 Jul;12(3):231-43
International journal of surgical pathology 2004 Jul;12(3):231-43
Oncocytic adrenocortical carcinomas: a pathological and immunohistochemical study of four cases in comparison with conventional adrenocortical carcinomas.
Song SY, Park S, Kim SR, Suh YL
Pathology international 2004 Aug;54(8):603-10
Pathology international 2004 Aug;54(8):603-10
Ectopic thyroid in the gallbladder: report of a case.
Ihtiyar E, Isiksoy S, Algin C, Sahin A, Erkasap S, Yasar B
Surgery today 2003;33(10):777-80
Surgery today 2003;33(10):777-80
Automated acquisition of stained tissue microarrays for high-throughput evaluation of molecular targets.
Vrolijk H, Sloos W, Mesker W, Franken P, Fodde R, Morreau H, Tanke H
The Journal of molecular diagnostics : JMD 2003 Aug;5(3):160-7
The Journal of molecular diagnostics : JMD 2003 Aug;5(3):160-7
Invasion of interstitial matrix by a novel cell line from primary peritoneal carcinosarcoma, and by established ovarian carcinoma cell lines: role of cell-matrix adhesion molecules, proteinases, and E-cadherin expression.
Kokenyesi R, Murray KP, Benshushan A, Huntley ED, Kao MS
Gynecologic oncology 2003 Apr;89(1):60-72
Gynecologic oncology 2003 Apr;89(1):60-72
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Supportive validation
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- Western blot analysis of Cytokeratin Pan was performed by loading 25 µg of A431 (Lane 1) and Hela (Lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Cytokeratin Pan monoclonal antibody (Product # MA5-13156) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 40-67 kDa.
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- Western blot was performed using Anti-Cytokeratin Pan Type I/II Antibody Cocktail (Product # MA5-13156) and a 40-60 kDa band corresponding to Cytokeratin Pan Type I/II was observed across all tested cell lines, except Raji. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), MCF7 (Lane 2), Caco-2 (Lane 3), Raji (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:10000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
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- Immunofluorescent analysis of Cytokeratin Pan (green) showing positive staining in the cytoplasm of MCF-7 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a Cytokeratin Pan monoclonal antibody (Product # MA5-13156) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
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- Immunofluorescence analysis of Cytokeratin Pan Type I/II was performed using 70% confluent log phase A-431 cells. The cells were fixed and permeabilized with ice-cold acetone at 4°C for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin Pan Type I/II Antibody Cocktail (Product # MA5-13156, 5 µg/mL) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoskeletal localization. Panel e represents Raji cells showing no expression of cytokeratin. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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- Formalin-fixed, paraffin-embedded human skin stained with Keratin, Pan antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells.
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- Immunohistochemistry analysis of Cytokeratin Pan showing positive staining in the cytoplasm of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cytokeratin Pan monoclonal antibody (Product # MA5-13156) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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- Immunohistochemistry analysis of Cytokeratin Pan showing positive staining in the cytoplasm of paraffin-treated Human lung squamous carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cytokeratin Pan monoclonal antibody (Product # MA5-13156) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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- Immunohistochemistry analysis of Cytokeratin Pan showing positive staining in the cytoplasm of paraffin-treated Mouse skin tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cytokeratin Pan monoclonal antibody (Product # MA5-13156) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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- Immunohistochemical analysis of pan Cytokeratin was performed using formalin-fixed paraffin-embedded human colon (neuro-endocrine tumor) tissue sections. To expose the target protein, heat-induced epitope retrieval (HIER) was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 20 minutes. Following antigen retrieval, the sections were blocked with 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without Cytokeratin Pan Type I/II Antibody Cocktail (Product # MA5-13156) at 2 µg/mL in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 in 0.1% normal goat serum for 45 minutes at room temperature. Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification.
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- Immunohistochemical analysis of pan Cytokeratin was performed using formalin-fixed paraffin-embedded mouse skin and mouse brain tissue sections. To expose the target protein, heat-induced epitope retrieval (HIER) was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 20 minutes. Following antigen retrieval, the sections were blocked with 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with Cytokeratin Pan Type I/II Antibody Cocktail (Product # MA5-13156) at 4 µg/mL in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 in 0.1% normal goat serum for 45 minutes at room temperature. Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification.
Supportive validation
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- FIGURE 1 Representative immunofluorescence images. (A) Representative image of a TMA core with heterogeneous infiltration of leukocytes in panel 1; red denoting CD68, yellow denoting CD56, green denoting CD3, pink denoting NKp46, cyan denoting CD163 and magenta denoting cytokeratin. (B) Representative image of a TMA core with heterogeneous infiltration of leukocytes in panel 2; green denoting CD1a, red denoting CD208, yellow denoting CD15, pink denoting CD123, cyan denoting CD68 and magenta denoting cytokeratin.
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- Fig. 5 Mucin production and epithelial phenotype in GECs. a. GEC were stained with PAS (reddish-purple staining indicates the presence of neutral mucins), b. MUC1 on GECs shows homogeneous cytoplasmic staining mainly located in the cell membrane, c. AGS cell lines (mucin positive control) and d. U-937 cell lines (negative control) e . Pan-cytokeratin markers AE1/AE3 on GECs: a positive fibrillar staining pattern distributed in the cytoplasm is observed, f. HeLa cell lines (positive control) and, g. U-937 cell lines (negative control). Images were visualized using conventional light microscopy. Images were taken at 20x magnification
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- Figure 3 M2 macrophages cause tumor cell budding from the colonosphere bulk (A) Representative images of bright field microscopy of the mono-cultures and co-cultures are shown in the upper panel. Green fluorescent images to identify tumor cells are shown in the middle panel, binarized images of the fluorescence are shown in the lower panel, 10x magnification on EVOS microscope; scale bars indicate 400 mum. (B) Adherence assay showing the number of small cell clusters (