10528-1-AP

antibody from Proteintech Group

Targeting: DDX21 GURDB, RH-II/GU

Provider product page for 10528-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [50]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 10528-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10528-1-AP, RRID:AB_2092705
Product name: DDX21 antibody
Antibody type: Polyclonal
Description: KD/KO validated DDX21 antibody (Cat. #10528-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

DDX21 protein structure - 10528-1-AP shown in red.

Submitted references Direct interaction between human DDX1 and SARS-CoV-2 nucleocapsid protein is regulated by phosphorylation.

Wang L, Baxley RM, Largaespada DA, Aihara H, Bielinsky AK
The Journal of biological chemistry 2026 Mar 26;302(5):111408

BARD1 recognizes pre-rRNA for DNA damage repair and rRNA biogenesis.

Huang H, Wu D, Yu X
The Journal of biological chemistry 2026 Mar 25;302(5):111406

DDX21 nuclear-cytoplasmic shuttling recruits RPS18 to viral dsRNA to promote CSFV replication.

Li B, Zhou B, Sun C, Li S, Bai Y, Zhao R, Song Y, He Y, Yi L, Ding H, Fan S, Chen J, Wu K
Veterinary microbiology 2026 Jan;312:110822

Pre-rRNA spatial distribution and functional organization of the nucleolus.

Pan YH, Shan L, Zhang YY, Yang ZH, Zhang Y, Cao SM, Liu XQ, Zhang J, Yang L, Chen LL
Nature 2025 Oct;646(8083):227-235

m6A-induced DEAD-box RNA helicase 21 enhances lipid metabolism via 3‑hydroxy-3-methylglutaryl-CoA synthases 1 in colorectal cancer.

Gao H, Zheng S, Liang J, Wang Y, Chen L, Li H, Chen Y, Zhang F, Shi H, Han A
Translational oncology 2025 May;55:102373

GPATCH4 functions as a regulator of nucleolar R-loops in hepatocellular carcinoma cells.

Zhao YM, Jiang Y, Wang JZ, Cao S, Zhu H, Wang WK, Yu J, Liu J, Hui J
Nucleic acids research 2025 May 22;53(10)

SART3 promotes homologous recombination repair by stimulating DNA-RNA hybrids removal and DNA end resection.

Fu H, Huang M, Wu H, Zheng H, Gong Y, Xing L, Gong J, An R, Li Q, Jie X, Ma X, Tang TS, Guo C
Nature communications 2025 Mar 6;16(1):2244

Human RPL7 and DDX21 interact with HTLV-1 Gag and enhance tRNA(Pro) primer annealing to genomic RNA.

Syu YC, Long Z, Musier-Forsyth K
bioRxiv : the preprint server for biology 2025 Jul 15;

CircRNA-RBAC induces cardiac repair by promoting ribosome biogenesis and cell cycle progression in cardiomyocytes.

Huang S, Wei G, Jia X, Tang Z, Chen Q, Li C, Yan W, Jin M, Li X, Chen Y, Zheng H, Chen G, Liao W, Liao Y, Wang Y, Li J, Bin J
International journal of biological macromolecules 2025 Jan;287:138406

DDX21 Controls Cell Cycle Progression and Autophagy in Pancreatic Cancer Cells.

Leccese A, Ruta V, Panzeri V, Attili F, Spada C, Cianfanelli V, Sette C
Cancers 2025 Feb 7;17(4)

mTOR signaling during T cell activation promotes cytokine production in T cells through 3' UTR-mediated translation control.

Jurgens AP, Zwijnen J, Bradarić A, van Alphen FPJ, Bresser K, Rooijers K, Hoogendijk AJ, Popović B, Wolkers MC
Molecular cell 2025 Dec 4;85(23):4452-4462.e5

DEAD-box protein 21 promotes renal fibrosis via p21-dependent cell cycle arrest in proximal tubular epithelial cells.

Tian M, Wang X, Zhang M, Li C, Xu Y, Chen X, Chen C, Wei Z, Li X, Ding G, Zhang L, Wang H, Gan H
Cellular signalling 2025 Apr;128:111654

Competitive binding between DDX21 and SIRT7 enhances NAT10-mediated ac(4)C modification to promote colorectal cancer metastasis and angiogenesis- DDX21 promotes colorectal cancer metastasis.

Song A, Liu B, Li W, Chen B, Gui P, Zhang H, Zhu C, Xu Y, Jiang T, Song J
Cell death & disease 2025 Apr 29;16(1):353

Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK.

Li Y, Munoz-Mayorga D, Nie Y, Kang N, Tao Y, Lagerwall J, Pernaci C, Curtin G, Coufal NG, Mertens J, Shi L, Chen X
Cell metabolism 2024 Jun 4;36(6):1351-1370.e8

Nucleolar Localization of the RNA Helicase DDX21 Predicts Survival Outcomes in Gynecologic Cancers.

Aljardali MW, Kremer KM, Parker JE, Fleming E, Chen H, Lea JS, Kraus WL, Camacho CV
Cancer research communications 2024 Jun 13;4(6):1495-1504

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice.

Zou D, Li K, Su L, Liu J, Lu Y, Huang R, Li M, Mang X, Geng Q, Li P, Tang J, Yu Z, Zhang Z, Chen D, Miao S, Yu J, Yan W, Song W
Developmental cell 2024 Jul 8;59(13):1707-1723.e8

Monoallelically expressed noncoding RNAs form nucleolar territories on NOR-containing chromosomes and regulate rRNA expression.

Hao Q, Liu M, Daulatabad SV, Gaffari S, Song YJ, Srivastava R, Bhaskar S, Moitra A, Mangan H, Tseng E, Gilmore RB, Frier SM, Chen X, Wang C, Huang S, Chamberlain S, Jin H, Korlach J, McStay B, Sinha S, Janga SC, Prasanth SG, Prasanth KV
eLife 2024 Jan 19;13

Downregulation of CPSF6 leads to global mRNA 3' UTR shortening and enhanced antiviral immune responses.

Ge Y, Huang J, Chen R, Fu Y, Ling T, Ou X, Rong X, Cheng Y, Lin Y, Zhou F, Lu C, Yuan S, Xu A
PLoS pathogens 2024 Feb;20(2):e1012061

DExD-Box Helicase 21 Enhances Myometrial Contractions Through Thrombospondin-1-Mediated Increase in Cell Adhesion.

Chen L, Jiang Y, Wang X, Wang L, Bao J, Lv Z, Sha X, Zheng Z, Chen Y, Ji K, Liu H
Journal of cellular and molecular medicine 2024 Dec;28(24):e70268

Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles.

Kamel W, Ruscica V, Embarc-Buh A, de Laurent ZR, Garcia-Moreno M, Demyanenko Y, Orton RJ, Noerenberg M, Madhusudhan M, Iselin L, Järvelin AI, Hannan M, Kitano E, Moore S, Merits A, Davis I, Mohammed S, Castello A
Molecular cell 2024 Dec 19;84(24):4896-4911.e7

The nucleolar phase of signal recognition particle assembly.

Issa A, Schlotter F, Flayac J, Chen J, Wacheul L, Philippe M, Sardini L, Mostefa L, Vandermoere F, Bertrand E, Verheggen C, Lafontaine DL, Massenet S
Life science alliance 2024 Aug;7(8)

Calmodulin Triggers Activity-Dependent rRNA Biogenesis via Interaction with DDX21.

Yang JL, Sun X, Shi JX, Cui QX, Cao XY, Wang KT, An MX, Wu SJ, Yang YL, Sun HZ, Zhao WD
The Journal of neuroscience : the official journal of the Society for Neuroscience 2024 Aug 28;44(35)

TRIP13 regulates progression of gastric cancer through stabilising the expression of DDX21.

Zhang G, Yang R, Wang B, Yan Q, Zhao P, Zhang J, Su W, Yang L, Cui H
Cell death & disease 2024 Aug 26;15(8):622

Decoding the genomic landscape of chromatin-associated biomolecular condensates.

Yu Z, Wang Q, Zhang Q, Tian Y, Yan G, Zhu J, Zhu G, Zhang Y
Nature communications 2024 Aug 13;15(1):6952

Lnc-PLCB1 is stabilized by METTL14 induced m6A modification and inhibits Helicobacter pylori mediated gastric cancer by destabilizing DDX21.

Chang M, Cui X, Sun Q, Wang Y, Liu J, Sun Z, Ren J, Sun Y, Han L, Li W
Cancer letters 2024 Apr 28;588:216746

The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1.

Cao L, Hui X, Xu T, Mao H, Lin X, Huang K, Zhao L, Jin M
Journal of immunology (Baltimore, Md. : 1950) 2023 Sep 15;211(6):1020-1031

Pirh2-dependent DNA damage in neurons induced by the G-quadruplex ligand pyridostatin.

Escarcega RD, Patil AA, Moruno-Manchon JF, Urayama A, Marrelli SP, Kim N, Monchaud D, McCullough LD, Tsvetkov AS
The Journal of biological chemistry 2023 Oct;299(10):105157

NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance.

Chen T, Xu ZG, Luo J, Manne RK, Wang Z, Hsu CC, Pan BS, Cai Z, Tsai PJ, Tsai YS, Chen ZZ, Li HY, Lin HK
Cell metabolism 2023 Oct 3;35(10):1782-1798.e8

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges.

Miyagi T, Ueda K, Sugimoto M, Yagi T, Ito D, Yamazaki R, Narumi S, Hayamizu Y, Uji-I H, Kuroda M, Kanekura K
iScience 2023 Jun 16;26(6):106957

Glucose dissociates DDX21 dimers to regulate mRNA splicing and tissue differentiation.

Miao W, Porter DF, Lopez-Pajares V, Siprashvili Z, Meyers RM, Bai Y, Nguyen DT, Ko LA, Zarnegar BJ, Ferguson ID, Mills MM, Jilly-Rehak CE, Wu CG, Yang YY, Meyers JM, Hong AW, Reynolds DL, Ramanathan M, Tao S, Jiang S, Flynn RA, Wang Y, Nolan GP, Khavari PA
Cell 2023 Jan 5;186(1):80-97.e26

2B and 3C Proteins of Senecavirus A Antagonize the Antiviral Activity of DDX21 via the Caspase-Dependent Degradation of DDX21.

Zhao K, Guo XR, Liu SF, Liu XN, Han Y, Wang LL, Lei BS, Zhang WC, Li LM, Yuan WZ
Frontiers in immunology 2022;13:951984

Pan-cancer analysis reveals DDX21 as a potential biomarker for the prognosis of multiple tumor types.

Hu A, Wang Y, Tian J, Chen Z, Chen R, Han X, Chen Y, Liu T, Chen Q
Frontiers in oncology 2022;12:947054

Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells.

Datta C, Truesdell SS, Wu KQ, Bukhari SIA, Ngue H, Buchanan B, Le Tonqueze O, Lee S, Kollu S, Granovetter MA, Boukhali M, Kreuzer J, Batool MS, Balaj L, Haas W, Vasudevan S
Science advances 2022 Oct 28;8(43):eabo1304

An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress.

Thompson L, Depledge DP, Burgess HM, Mohr I
Cell reports 2022 May 3;39(5):110767

The Roles of RNA Helicases in DNA Damage Repair and Tumorigenesis Reveal Precision Therapeutic Strategies.

Xie J, Wen M, Zhang J, Wang Z, Wang M, Qiu Y, Zhao W, Zhu F, Yao M, Rong Z, Hu W, Pei Q, Sun X, Li J, Mao Z, Sun LQ, Tan R
Cancer research 2022 Mar 1;82(5):872-884

Nuclear microRNAs release paused Pol II via the DDX21-CDK9 complex.

Ohno SI, Oikawa K, Tsurui T, Harada Y, Ono K, Tateishi M, Mirza A, Takanashi M, Kanekura K, Nagase K, Shimada Y, Kudo Y, Ikeda N, Ochiya T, Wang X, Kuroda M
Cell reports 2022 Apr 12;39(2):110673

lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.

Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang ZH, Shan L, Yang L, Liu J, Chen LL
Science (New York, N.Y.) 2021 Jul 30;373(6554):547-555

Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics.

Zhang S, Hinde E, Parkyn Schneider M, Jans DA, Bogoyevitch MA
Scientific reports 2020 Jan 31;10(1):1557

PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.

Johansson JA, Marie KL, Lu Y, Brombin A, Santoriello C, Zeng Z, Zich J, Gautier P, von Kriegsheim A, Brunsdon H, Wheeler AP, Dreger M, Houston DR, Dooley CM, Sims AH, Busch-Nentwich EM, Zon LI, Illingworth RS, Patton EE
Developmental cell 2020 Aug 10;54(3):317-332.e9

Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.

Kim DS, Camacho CV, Nagari A, Malladi VS, Challa S, Kraus WL
Molecular cell 2019 Sep 19;75(6):1270-1285.e14

Dissecting the Role of DDX21 in Regulating Human Cytomegalovirus Replication.

Hao H, Han T, Xuan B, Sun Y, Tang S, Yue N, Qian Z
Journal of virology 2019 Dec 15;93(24)

A Survey of DDX21 Activity During Rev/RRE Complex Formation.

Hammond JA, Zhou L, Lamichhane R, Chu HY, Millar DP, Gerace L, Williamson JR
Journal of molecular biology 2018 Feb 16;430(4):537-553

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.

Xing YH, Yao RW, Zhang Y, Guo CJ, Jiang S, Xu G, Dong R, Yang L, Chen LL
Cell 2017 May 4;169(4):664-678.e16

DDX21 translocates from nucleus to cytoplasm and stimulates the innate immune response due to dengue virus infection.

Dong Y, Ye W, Yang J, Han P, Wang Y, Ye C, Weng D, Zhang F, Xu Z, Lei Y
Biochemical and biophysical research communications 2016 Apr 29;473(2):648-53

Identification of C/EBPβ target genes in ALK+ anaplastic large cell lymphoma (ALCL) by gene expression profiling and chromatin immunoprecipitation.

Bonzheim I, Irmler M, Klier-Richter M, Steinhilber J, Anastasov N, Schäfer S, Adam P, Beckers J, Raffeld M, Fend F, Quintanilla-Martinez L
PloS one 2013;8(5):e64544

A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness.

Grau L, Luque-Garcia JL, González-Peramato P, Theodorescu D, Palou J, Fernandez-Gomez JM, Sánchez-Carbayo M
PloS one 2013;8(1):e53328

Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21.

Hirai Y, Louvet E, Oda T, Kumeta M, Watanabe Y, Horigome T, Takeyasu K
Genes to cells : devoted to molecular & cellular mechanisms 2013 Sep;18(9):780-97

Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA.

Hochstatter J, Hölzel M, Rohrmoser M, Schermelleh L, Leonhardt H, Keough R, Gonda TJ, Imhof A, Eick D, Längst G, Németh A
The Journal of biological chemistry 2012 Jul 13;287(29):24365-77

Differential effects of AKT1(p.E17K) expression on human mammary luminal epithelial and myoepithelial cells.

Salhia B, Van Cott C, Tegeler T, Polpitiya A, Duquette RA, Gale M, Hostteter G, Petritis K, Carpten J
Human mutation 2012 Aug;33(8):1216-27

Differential protein expression profiling by iTRAQ-two-dimensional LC-MS/MS of human bladder cancer EJ138 cells transfected with the metastasis suppressor KiSS-1 gene.

Ruppen I, Grau L, Orenes-Piñero E, Ashman K, Gil M, Algaba F, Bellmunt J, Sánchez-Carbayo M
Molecular & cellular proteomics : MCP 2010 Oct;9(10):2276-91

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 10528-1-AP(DDX21 antibody) at dilution of 1:2000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using DDX21 antibody 10528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 10528-1-AP(DDX21 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue