GTX133367

antibody from GeneTex

Targeting: SNCA NACP, PARK1, PARK4, PD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX133367

Provider

GeneTex

Product name

alpha Synuclein antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX133367) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various tissue extracts (50 ?g) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody (GTX133367) diluted at 1:2000 and competitor's antibody (MO22112) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various tissue extracts (50 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX133367) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX133367) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX133367) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX133367) diluted at 1:250.Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.