Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 848601 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#848601, RRID:AB_2650693
- Product name
- Purified anti-α-Synuclein, 117-122
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- A15126D
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot of anti-α-Synuclein antibody (clone A15126D) and isotype-matched IgG2b control, binding to endogenous and recombinant human α-synuclein. Lane 1: 50 ng of recombinant human α-synuclein; Lane 2: 50 ng of recombinant C-terminally truncated human α-synuclein (1-122). Lane 3: 20 µg of normal human brain lysate. Blots were incubated with 1 µg/ml of each primary antibody at room temperature for two hours, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enhanced chemiluminescence was used as the detection system.
- Conjugate
- Unconjugated