LS-C773113

antibody from LSBio

Targeting: SNCA NACP, PARK1, PARK4, PD1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C773113

Provider

LSBio

Product name

SNCA / Alpha-Synuclein Antibody (clone 3C11) LS-C773113

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3C11

Storage

Store at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: cerebellum. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Alpha Synuclein Antibody (D) Composite.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: cerebellum. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Alpha Synuclein Antibody (D) Composite.