Ab185363

antibody from Aladdin Scientific

Targeting: SNCA NACP, PARK1, PARK4, PD1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab185363

Provider

Aladdin Scientific

Product name

Alpha-synuclein Mouse mAb

Antibody type

Monoclonal

Description

ApplicationWB: 1/1,000 - 1/200,000; Predicted molecular weight: 14.5 KDaFlow: 1/200 - 1/400

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

GH03/3B5

Vial size

100_l,10_l,1ml,50_l

Concentration

1 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Avoid freeze / thaw cycle .

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha-synuclein Mouse mAb (Ab185363) - Western Blot All lanes: Alpha-synuclein Mouse Antibody (Ab185363) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 17 kDa Exposure time: 4.5 s

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha-synuclein Mouse mAb (Ab185363) - Flow Cytometry Overlay histogram showing HeLa cells stained with Alpha-synuclein Mouse mAb (Ab185363) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).