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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP1-26380 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-26380, RRID:AB_1853504
- Product name
- Mouse Monoclonal alpha-Synuclein Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
2 nitrated residues Y125, Y133- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references An Enriched Environment Ameliorates Oxidative Stress and Olfactory Dysfunction in Parkinson's Disease with α-Synucleinopathy.
Determining nuclear localization of alpha-synuclein in mouse brains.
Oxidative damage linked to neurodegeneration by selective alpha-synuclein nitration in synucleinopathy lesions.
Oxidative damage linked to neurodegeneration by selective alpha-synuclein nitration in synucleinopathy lesions.
Wi S, Lee JW, Kim M, Park CH, Cho SR
Cell transplantation 2018 May;27(5):831-839
Cell transplantation 2018 May;27(5):831-839
Determining nuclear localization of alpha-synuclein in mouse brains.
Huang Z, Xu Z, Wu Y, Zhou Y
Neuroscience 2011 Dec 29;199:318-32
Neuroscience 2011 Dec 29;199:318-32
Oxidative damage linked to neurodegeneration by selective alpha-synuclein nitration in synucleinopathy lesions.
Giasson BI, Duda JE, Murray IV, Chen Q, Souza JM, Hurtig HI, Ischiropoulos H, Trojanowski JQ, Lee VM
Science (New York, N.Y.) 2000 Nov 3;290(5493):985-9
Science (New York, N.Y.) 2000 Nov 3;290(5493):985-9
Oxidative damage linked to neurodegeneration by selective alpha-synuclein nitration in synucleinopathy lesions.
Giasson BI, Duda JE, Murray IV, Chen Q, Souza JM, Hurtig HI, Ischiropoulos H, Trojanowski JQ, Lee VM
Science (New York, N.Y.) 2000 Nov 3;290(5493):985-9
Science (New York, N.Y.) 2000 Nov 3;290(5493):985-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - (A) Nitrated Synuclein-alpha protein and (B) Non-nitrated Synuclein-alpha protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - Analysis of FFPE human brain using alpha-Synuclein antibody (clone 24.8) at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic puncta and varicosities staining was observed. Staining was performed by Histowiz.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using Synuclein-alpha [Nitrate Tyr125, Nitrate Tyr133] antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
