Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- NB100-373 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-373, RRID:AB_2192488
- Product name
- Rabbit Polyclonal SMC2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Nesprin-2 Interacts with Condensin Component SMC2.
PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.
Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response.
PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture.
Differential regulation of telomere and centromere cohesion by the Scc3 homologues SA1 and SA2, respectively, in human cells.
Identification of cellular proteins that interact with the adeno-associated virus rep protein.
Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage.
Xing X, Mroß C, Hao L, Munck M, Herzog A, Mohr C, Unnikannan CP, Kelkar P, Noegel AA, Eichinger L, Neumann S
International journal of cell biology 2017;2017:8607532
International journal of cell biology 2017;2017:8607532
PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.
Liu W, Tanasa B, Tyurina OV, Zhou TY, Gassmann R, Liu WT, Ohgi KA, Benner C, Garcia-Bassets I, Aggarwal AK, Desai A, Dorrestein PC, Glass CK, Rosenfeld MG
Nature 2010 Jul 22;466(7305):508-12
Nature 2010 Jul 22;466(7305):508-12
Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response.
Bandhakavi S, Kim YM, Ro SH, Xie H, Onsongo G, Jun CB, Kim DH, Griffin TJ
Molecular & cellular proteomics : MCP 2010 Feb;9(2):403-14
Molecular & cellular proteomics : MCP 2010 Feb;9(2):403-14
PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture.
Kurasawa Y, Yu-Lee LY
Molecular biology of the cell 2010 Apr 1;21(7):1188-99
Molecular biology of the cell 2010 Apr 1;21(7):1188-99
Differential regulation of telomere and centromere cohesion by the Scc3 homologues SA1 and SA2, respectively, in human cells.
Canudas S, Smith S
The Journal of cell biology 2009 Oct 19;187(2):165-73
The Journal of cell biology 2009 Oct 19;187(2):165-73
Identification of cellular proteins that interact with the adeno-associated virus rep protein.
Nash K, Chen W, Salganik M, Muzyczka N
Journal of virology 2009 Jan;83(1):454-69
Journal of virology 2009 Jan;83(1):454-69
Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage.
Blank M, Lerenthal Y, Mittelman L, Shiloh Y
The Journal of cell biology 2006 Jul 17;174(2):195-206
The Journal of cell biology 2006 Jul 17;174(2):195-206
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.