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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- A301-593A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCAD1/ETL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMARCAD1/ETL1 by western blot. Samples: Whole cell lysate (50 µg) from MDA-MB-231, MCF-7, HEK293T, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCAD1/ETL1 antibody (Product # A301-593A; lot A301-593A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SMARCAD1/ETL1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SMARCAD1/ETL1 antibody A301-593A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. SMARCAD1/ETL1 was also immunoprecipitated by rabbit anti-SMARCAD1/ETL1 antibody A301-592A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMARCAD1/ETL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCAD1/ETL1 antibody (Product # A301-593A; lot A301-593A-1) used for IP at 6 µg per reaction. SMARCAD1/ETL1 was also immunoprecipitated by rabbit anti-SMARCAD1/ETL1 antibody (Product # A301-592A). For blotting immunoprecipitated SMARCAD1/ETL1, Product # A301-593A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
