RQ5189

antibody from NSJ Bioreagents

Targeting: SMARCAD1 DKFZP762K2015, ETL1, KIAA1122

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5189

Provider

NSJ Bioreagents

Product name

SMARCAD1 Antibody

Antibody type

Polyclonal

Description

This highly specific SMARCAD1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Immunocytochemistry/Flow cytometry/Immunoprecipitation/Direct ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

Store the SMARCAD1 antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) K562, 2) HL-60, 3) U-2 OS, 4) U-87 MG, 5) HepG2, 6) ThP-1 and 7) A549 lysate with SMARCAD1 antibody. Predicted molecular weight ~117 kDa but can be observed at up to ~150 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat testis and mouse 2) thymus, 3) lung, 4) testis and 5) kidney tissue lysate with SMARCAD1 antibody. Predicted molecular weight ~117 kDa but can be observed at up to ~150 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human A431 cells with SMARCAD1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunoprecipitation

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunoprecipitation of SMARCAD1 protein from 500ug of human K562 whole cell lysate with 2ug of SMARCAD1 antibody.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human breast cancer with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon cancer with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse brain with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat brain with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human SiHa cells with SMARCAD1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMARCAD1 antibody.