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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- MA5-38074 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HRH3 Recombinant Rabbit Monoclonal Antibody (7H8E6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: Mouse testis, Mouse brain, Mouse spleen, Rat testis, Rat brain, Rat spleen, Raji, SH-SY5Y, U-251MG Immunogen sequence: MERAPPDGPL NASGALAGEA AAAGGARGFS AAWTAVLAAL MALLIVATVL GNALVMLAFV ADSSLRTQNN FFLLNLAISD FLVGAFCIPL YVPYVLTGRW
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 7H8E6
- Vial size
- 100 µL
- Concentration
- 0.74 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HRH3 in extracts of various cell lines. Samples were incubated with HRH3 Monoclonal antibody (Product # MA5-38074) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HRH3 in extracts of various cell lines. Samples were incubated with HRH3 Monoclonal antibody (Product # MA5-38074) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HRH3 in 300 μg extracts of Raji cells. Samples were precipitated with 3 μg HRH3 Monoclonal antibody (Product # MA5-38074). Western blot was performed from the immunoprecipitate using HRH3 Monoclonal antibody (Product # MA5-38074) at a dilution of 1:1,000.
