Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32697 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUT3 Recombinant Rabbit Monoclonal Antibody (JA50-31)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JA50-31
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inhibition of PFKFB3 Hampers the Progression of Atherosclerosis and Promotes Plaque Stability.
Poels K, Schnitzler JG, Waissi F, Levels JHM, Stroes ESG, Daemen MJAP, Lutgens E, Pennekamp AM, De Kleijn DPV, Seijkens TTP, Kroon J
Frontiers in cell and developmental biology 2020;8:581641
Frontiers in cell and developmental biology 2020;8:581641
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT3 in different lysates using a Monoclonal antibody (Product #MA5-32697) at a dilution of 1:1,000. Positive control: Lane1: Human lung, Lane 2: HepG2, Lane 3: Mouse heart.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT3 in different lysates using a Monoclonal antibody (Product #MA5-32697) at a dilution of 1:1,000. Positive control: Lane1: mouse spleen tissue, Lane 2: HepG2, Lane 3: mouse heart tissue, Lane 4: rat heart tissue.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GLUT3 Recombinant Rabbit Monoclonal Antibody (JA50-31) (Product # MA5-32697) and a 53 kDa band corresponding to GLUT3 was observed across cell lines and tissue extracts tested except Mouse Skeletal Muscle and Rat Skeletal Muscle which are reported to be low. An uncharacterized band was observed between 40-50 kDa in the panel tested. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), BeWo (Lane 2), Caco-2 (Lane 3), Hep G2 (Lane 4), NTERA-2 cl.D1 (Lane 5), MCF-7 (Lane 6), HEK-293 (Lane 7), tissue extracts of Mouse Skeletal Muscle (Lane 8), Rat Skeletal Muscle (Lane 9), Mouse Brain (Lane 10) and Rat Brain (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GLUT3 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GLUT3 Rabbit Recombinant Monoclonal Antibody (JA50-31) (Product # MA5-32697) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLUT3 of paraffin-embedded Human brain tissue using a GLUT3 Monoclonal antibody (Product #MA5-32697). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLUT3 of paraffin-embedded Human embryo tissue using a GLUT3 Monoclonal antibody (Product #MA5-32697). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLUT3 of paraffin-embedded Mouse brain tissue using a GLUT3 Monoclonal antibody (Product #MA5-32697). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of GLUT3 in SH-SY5Y cells using a GLUT3 Monoclonal Antibody (Product # MA5-32697) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).