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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [3]
- Immunohistochemistry [9]
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- Product number
- PA5-99486 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUT3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total GLUT3.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT3 in extracts from various samples. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486). Lane 1: Mouse lung, blocked with antigen-specific peptides; Lane 2: Mouse lung; Lane 3: Mouse brain; Lane 4: Hepg2 cells (serum starvation treatment); Lane 5: Hela cells (LPS 4h treatment).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT3 in extracts from various samples. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486). Lane 1: A549 cells (serum starvation treatment), blocked with antigen-specific peptides; Lane 2: A549 cells (serum starvation treatment); Lane 3: K562 cells (serum starvation treatment); Lane 4: 293 cells (serum starvation treatment); Lane 5: Raw264.7 cells (UV treatment).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT3 in HUVEC cell lysate (left lane: treated with blocking peptide). Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT3 in MCF7 cell lysate (left lane: treated with blocking peptide). Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT3 in mouse brain tissue (Lane 1: treated with blocking peptide). Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GLUT3 in LOVO. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GLUT3 in LOVO. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GLUT3 in LOVO. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLUT3 in human kidney cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLUT3 in mouse kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLUT3 in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLUT3 in rat kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLUT3 in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLUT3 in human kidney cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLUT3 in mouse kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLUT3 in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLUT3 in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GLUT3 polyclonal antibody (Product # PA5-99486) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
