Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- PA5-68506 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAX7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Cow: 100%; Dog: 100%; Goat: 86%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat.
Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat.
Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat.
Stout AJ, Mirliani AB, Rittenberg ML, Shub M, White EC, Yuen JSK Jr, Kaplan DL
Communications biology 2022 Jun 2;5(1):466
Communications biology 2022 Jun 2;5(1):466
Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat.
Park S, Gagliardi M, Swennen G, Dogan A, Kim Y, Park Y, Park G, Oh S, Post M, Choi J
Biomolecules 2022 Jun 16;12(6)
Biomolecules 2022 Jun 16;12(6)
Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat.
Simsa R, Yuen J, Stout A, Rubio N, Fogelstrand P, Kaplan DL
Foods (Basel, Switzerland) 2019 Oct 21;8(10)
Foods (Basel, Switzerland) 2019 Oct 21;8(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PAX7 Polyclonal Antibody (Product # PA5-68506) and a 54 kDa band corresponding to PAX7 was observed only in Mouse heart and Rat heart in comparison to other tissues which are reported to be low. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Brain (Lane 3), Rat Brain (Lane 4), Mouse Kidney (Lane 5), Rat Kidney (Lane 6), Mouse Liver (Lane 7) and Rat Liver (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PAX7 in C2C12 cells using PAX7 polyclonal antibody (Product # PA5-68506).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PAX7 in C2C12 cells using PAX7 polyclonal antibody (Product # PA5-68506).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for DAPI, actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Scale bars are 200 um.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The cell nuclei stained with Hoechst (Hoechst 33,342 nucleic acid stain) were blue fluorescence and Pax7 and MyoD proteins were stained with green fluorescence. ( A ): The nuclei and MyoD protein of Hanwoo myosatellite cells cultured for 1 day in hypoxia (2% O 2 ) and normoxia (20% O 2 ) were stained. ( B , C ): The nuclei, Pax7 and MyoD protein of Hanwoo myosatellite cells cultured for 3 days in hypoxia (2% O 2 ) and normoxia (20% O 2 ) were stained. Experiments were performed in triplicate and repeated three times (* p < 0.05). Hanwoo myosatellite cells were seeded in T25 flasks at 1800 cells/cm 2 and cultured in GM for 6 days in normoxia (20% O 2 ) or hypoxia (2% O 2 ) ( D ). ( E ): Relative Pax7, Myf5, MyoD and HIF1alpha mRNA levels were compared in Hanwoo myosatellite cells from ( D ). GAPDH was used as an internal control for RT-PCR. scale bar: 100 mum.