Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C745393 - Provider product page
- Provider
- LSBio
- Product name
- PAX7 Antibody (Internal) LS-C745393
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit anti-Pax7 antibody. Lane 1: Opal Pre-stained ladder Lane 2: Mouse muscle protein lysate. Lane 3: C2C12. Lane 4: Ms spleen. Lane 5: Ms brain. Lane 6: HEK293T. Lane 7: HeLa WCL. Lane 8: MCF7. Lane 9: Jurkat. Lane 10: A431. Lane 11: A549. Lane 12: LNCap. Lane 13: MOLT-4. Lane 14: Ramos. Lane 15: Raji. Lane 16: Opal Pre-stained ladder Load: 10 µg per lane. Primary antibody: Pax7 antibody at 1:1,000 for overnight at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa for Pax7.