LS-C747822

antibody from LSBio

Targeting: PAX7 Hup1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C747822

Provider

LSBio

Product name

PAX7 Antibody LS-C747822

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of 293T cells, using PAX7 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of 293T cells, using PAX7 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain using PAX7 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain using PAX7 antibody at dilution of 1:100 (40x lens).