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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-23990 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAX7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a PAX7 polyclonal antibody (Product # PA5-23990) in mouse spleen tissue lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using PAX7 Polyclonal Antibody (Product # PA5-23990) and a 55 kDa band corresponding to PAX7 was observed in cell lines and tissue tested. Whole cell extracts (30 µg lysate) of A-673 (Lane 1), RD (Lane 2), C2C12 (Lane 3), C2C12 differentiated to myotubes (Lane 4), Mouse Heart (Lane 5), Mouse Liver (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). PAX7 is highly expressed in A673, RD; C2C12 when compared to undifferentiated C2C12 and mouse heart when compared to mouse liver. Additional uncharacterized bands were observed across samples tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human spleen tissue using a PAX7 polyclonal antibody (Product # PA5-23990), followed by HRP-conjugated secondary antibody and DAB staining.
