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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [24]
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- Validations
- Immunocytochemistry [2]
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- Product number
- MAB1675 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat/Chicken Pax7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, rat, and chicken Pax7 (3-8).
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- PAX7
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tbx1 regulates inherited metabolic and myogenic abilities of progenitor cells derived from slow- and fast-type muscle.
The natural involution of the sheep proximal sesamoidean ligament is due to depletion of satellite cells and simultaneous proliferation of fibroblasts: Ultrastructural evidence.
Effect of 8-week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats.
Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line.
Recovery from impaired muscle growth arises from prolonged postnatal accretion of myonuclei in Atrx mutant mice.
Prenatal myonuclei play a crucial role in skeletal muscle hypertrophy in rodents.
Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice.
CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration.
Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness.
A Gingiva-Derived Mesenchymal Stem Cell-Laden Porcine Small Intestinal Submucosa Extracellular Matrix Construct Promotes Myomucosal Regeneration of the Tongue.
Generation of thalamic neurons from mouse embryonic stem cells.
Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease.
Identification of FHL1 as a therapeutic target for Duchenne muscular dystrophy.
Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
RAGE signaling deficiency in rhabdomyosarcoma cells causes upregulation of PAX7 and uncontrolled proliferation.
Satellite cells senescence in limb muscle of severe patients with COPD.
Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells.
A simplified but robust method for the isolation of avian and mammalian muscle satellite cells.
Skeletal muscle regeneration in Xenopus tadpoles and zebrafish larvae.
Sparing of extraocular muscle in aging and muscular dystrophies: a myogenic precursor cell hypothesis.
Extracellular matrix components direct porcine muscle stem cell behavior.
Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells.
Efficient conversion of ES cells into myogenic lineage using the gene-inducible system.
Skeletal myogenesis by human embryonic stem cells.
Motohashi N, Uezumi A, Asakura A, Ikemoto-Uezumi M, Mori S, Mizunoe Y, Takashima R, Miyagoe-Suzuki Y, Takeda S, Shigemoto K
Cell death and differentiation 2019 Jun;26(6):1024-1036
Cell death and differentiation 2019 Jun;26(6):1024-1036
The natural involution of the sheep proximal sesamoidean ligament is due to depletion of satellite cells and simultaneous proliferation of fibroblasts: Ultrastructural evidence.
Melotti L, Vezzoli E, Mascarello F, Maccatrozzo L, Patruno M
Research in veterinary science 2019 Jun;124:106-111
Research in veterinary science 2019 Jun;124:106-111
Effect of 8-week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats.
Lim CH, Gil JH, Quan H, Viet DH, Kim CK
Physiological reports 2018 Jun;6(12):e13725
Physiological reports 2018 Jun;6(12):e13725
Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line.
Kitajima Y, Ono Y
Skeletal muscle 2018 Aug 24;8(1):26
Skeletal muscle 2018 Aug 24;8(1):26
Recovery from impaired muscle growth arises from prolonged postnatal accretion of myonuclei in Atrx mutant mice.
Huh MS, Young KG, Yan K, Price-O'Dea T, Picketts DJ
PloS one 2017;12(11):e0186989
PloS one 2017;12(11):e0186989
Prenatal myonuclei play a crucial role in skeletal muscle hypertrophy in rodents.
Kawano F, Ono Y, Fujita R, Watanabe A, Masuzawa R, Shibata K, Hasegawa S, Nakata K, Nakai N
American journal of physiology. Cell physiology 2017 Mar 1;312(3):C233-C243
American journal of physiology. Cell physiology 2017 Mar 1;312(3):C233-C243
Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice.
Colaianni G, Mongelli T, Cuscito C, Pignataro P, Lippo L, Spiro G, Notarnicola A, Severi I, Passeri G, Mori G, Brunetti G, Moretti B, Tarantino U, Colucci SC, Reseland JE, Vettor R, Cinti S, Grano M
Scientific reports 2017 Jun 6;7(1):2811
Scientific reports 2017 Jun 6;7(1):2811
CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration.
Maeda Y, Yonemochi Y, Nakajyo Y, Hidaka H, Ikeda T, Ando Y
Scientific reports 2017 Jun 12;7(1):3305
Scientific reports 2017 Jun 12;7(1):3305
Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness.
Pryce BR, Al-Zahrani KN, Dufresne S, Belkina N, Labrèche C, Patino-Lopez G, Frenette J, Shaw S, Sabourin LA
Skeletal muscle 2017 Feb 2;7(1):3
Skeletal muscle 2017 Feb 2;7(1):3
A Gingiva-Derived Mesenchymal Stem Cell-Laden Porcine Small Intestinal Submucosa Extracellular Matrix Construct Promotes Myomucosal Regeneration of the Tongue.
Xu Q, Shanti RM, Zhang Q, Cannady SB, O'Malley BW Jr, Le AD
Tissue engineering. Part A 2017 Apr;23(7-8):301-312
Tissue engineering. Part A 2017 Apr;23(7-8):301-312
Generation of thalamic neurons from mouse embryonic stem cells.
Shiraishi A, Muguruma K, Sasai Y
Development (Cambridge, England) 2017 Apr 1;144(7):1211-1220
Development (Cambridge, England) 2017 Apr 1;144(7):1211-1220
Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease.
Thériault ME, Paré MÈ, Lemire BB, Maltais F, Debigaré R
Respiratory research 2014 Mar 25;15:35
Respiratory research 2014 Mar 25;15:35
Identification of FHL1 as a therapeutic target for Duchenne muscular dystrophy.
D'Arcy CE, Feeney SJ, McLean CA, Gehrig SM, Lynch GS, Smith JE, Cowling BS, Mitchell CA, McGrath MJ
Human molecular genetics 2014 Feb 1;23(3):618-36
Human molecular genetics 2014 Feb 1;23(3):618-36
Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
Brisson BK, Spinazzola J, Park S, Barton ER
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E965-74
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E965-74
RAGE signaling deficiency in rhabdomyosarcoma cells causes upregulation of PAX7 and uncontrolled proliferation.
Riuzzi F, Sorci G, Sagheddu R, Sidoni A, Alaggio R, Ninfo V, Donato R
Journal of cell science 2014 Apr 15;127(Pt 8):1699-711
Journal of cell science 2014 Apr 15;127(Pt 8):1699-711
Satellite cells senescence in limb muscle of severe patients with COPD.
Thériault ME, Paré MÈ, Maltais F, Debigaré R
PloS one 2012;7(6):e39124
PloS one 2012;7(6):e39124
Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells.
Awaya T, Kato T, Mizuno Y, Chang H, Niwa A, Umeda K, Nakahata T, Heike T
PloS one 2012;7(12):e51638
PloS one 2012;7(12):e51638
A simplified but robust method for the isolation of avian and mammalian muscle satellite cells.
Baquero-Perez B, Kuchipudi SV, Nelli RK, Chang KC
BMC cell biology 2012 Jun 21;13:16
BMC cell biology 2012 Jun 21;13:16
Skeletal muscle regeneration in Xenopus tadpoles and zebrafish larvae.
Rodrigues AM, Christen B, Martí M, Izpisúa Belmonte JC
BMC developmental biology 2012 Feb 27;12:9
BMC developmental biology 2012 Feb 27;12:9
Sparing of extraocular muscle in aging and muscular dystrophies: a myogenic precursor cell hypothesis.
Kallestad KM, Hebert SL, McDonald AA, Daniel ML, Cu SR, McLoon LK
Experimental cell research 2011 Apr 1;317(6):873-85
Experimental cell research 2011 Apr 1;317(6):873-85
Extracellular matrix components direct porcine muscle stem cell behavior.
Wilschut KJ, Haagsman HP, Roelen BA
Experimental cell research 2010 Feb 1;316(3):341-52
Experimental cell research 2010 Feb 1;316(3):341-52
Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells.
Shi M, Ishikawa M, Kamei N, Nakasa T, Adachi N, Deie M, Asahara T, Ochi M
Stem cells (Dayton, Ohio) 2009 Apr;27(4):949-60
Stem cells (Dayton, Ohio) 2009 Apr;27(4):949-60
Efficient conversion of ES cells into myogenic lineage using the gene-inducible system.
Ozasa S, Kimura S, Ito K, Ueno H, Ikezawa M, Matsukura M, Yoshioka K, Araki K, Yamamura KI, Abe K, Niwa H, Miike T
Biochemical and biophysical research communications 2007 Jun 15;357(4):957-63
Biochemical and biophysical research communications 2007 Jun 15;357(4):957-63
Skeletal myogenesis by human embryonic stem cells.
Zheng JK, Wang Y, Karandikar A, Wang Q, Gai H, Liu AL, Peng C, Sheng HZ
Cell research 2006 Aug;16(8):713-22
Cell research 2006 Aug;16(8):713-22
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Pax7 in C2C12 Mouse Cell Line. Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Pax7 in iBJ6 iPS Cell Line. Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
