500-P237-100UG

antibody from Invitrogen Antibodies

Targeting: CXCL14 BMAC, bolekine, BRAK, Kec, KS1, MIP-2g, NJAC, SCYB14

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P237-100UG

Provider

Invitrogen Antibodies

Product name

CXCL14 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BRAK (CXCL14). Anti-Human BRAK (CXCL14)-specific antibody was purified by affinity chromatography employing an immobilized Human BRAK (CXCL14) matrix. Sandwich ELISA: To detect Human BRAK (CXCL14) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BRAK (CXCL14) (500-P237Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BRAK (CXCL14). Western Blot: To detect Human BRAK (CXCL14) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BRAK (CXCL14) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Reactivity

Human

Host

Rabbit

Vial size

100 µg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

References

Submitted references

CXCL14-like Immunoreactivity Exists in Somatostatin-containing Endocrine Cells, and in the Lamina Propria and Submucosal Somatostatinergic Nervous System of Mouse Alimentary Tract.

Suzuki H, Yamada K, Matsuda Y, Onozuka M, Yamamoto T
Acta histochemica et cytochemica 2017 Dec 26;50(6):149-158

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CXCL14-Like Immunoreactivity Exists in Somatostatin-Containing Cells of Mouse Pancreas.

Suzuki H, Yamamoto T
Acta histochemica et cytochemica 2015 Dec 25;48(6):173-8

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human BRAK (CXCL14) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CXCL14 Polyclonal Antibody (Product # 500-P237-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CXCL14 Polyclonal Antibody, Biotin (Product # 500-P237BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BRAK (CXCL14).