Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-7098-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-9 Monoclonal Antibody (MH9D1), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody MH9D1 recognizes interleukin-9 (IL-9), a proinflammatory cytokine historically believed to be involved in type 2 immune responses. Recent evidence suggests IL-9 may instead be secreted by a distinct T-helper lineage called Th9. These cells can be derived from Th2 cells with TGF beta or differentiated directly from naive CD4+ T cells with TGF beta and IL-4. IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. Staining with the MH9D1 antibody is not blocked by the MH9A4 antibody, suggesting that the two antibodies bind to unique epitopes. Applications Reported: This MH9D1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MH9D1 antibody has been pre-titrated and tested on restimulated Th2-polarized normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- MH9D1
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Increased circulating IL-9-producing CD8+ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.
Proteinase 3 on apoptotic cells disrupts immune silencing in autoimmune vasculitis.
Wang W, Cheng ZS, Chen YF, Lin YH
Experimental and therapeutic medicine 2016 Dec;12(6):4055-4060
Experimental and therapeutic medicine 2016 Dec;12(6):4055-4060
Proteinase 3 on apoptotic cells disrupts immune silencing in autoimmune vasculitis.
Millet A, Martin KR, Bonnefoy F, Saas P, Mocek J, Alkan M, Terrier B, Kerstein A, Tamassia N, Satyanarayanan SK, Ariel A, Ribeil JA, Guillevin L, Cassatella MA, Mueller A, Thieblemont N, Lamprecht P, Mouthon L, Perruche S, Witko-Sarsat V
The Journal of clinical investigation 2015 Nov 2;125(11):4107-21
The Journal of clinical investigation 2015 Nov 2;125(11):4107-21
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Th9-polarized normal human peripheral blood cells were stimulated with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) and then intracellularly stained with Anti-Human CD4 FITC (Product # 11-0049-42) and Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or Anti-Human IL-9 PE (right) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye