507604

antibody from BioLegend

Targeting: IL9 HP40, IL-9, P40

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

507604

Provider

BioLegend

Proper citation

BioLegend Cat#507604, RRID:AB_315486

Product name

PE anti-human IL-9

Antibody type

Monoclonal

Antigen

Baculovirus-expressed, recombinant human IL-9

Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 has also been reported to protect human T cells from apoptosis induced by IL-2 withdrawal. IL-9 is upregulated in human eosinophils by TNF-α and IL1-β. IL-9 has been reported to downregulate the oxidative burst in activated human alveolar macrophages and induce TGF-β production. The recently characterized Th-9 helper cell lineage is characterized by the production of IL-9. The MH9A4 antibody reacts with human IL-9. The MH9A4 antibody can neutralize the bioactivity of natural or recombinant IL-9.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Vial size

25 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Submitted references

The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation.

Chang HC, Sehra S, Goswami R, Yao W, Yu Q, Stritesky GL, Jabeen R, McKinley C, Ahyi AN, Han L, Nguyen ET, Robertson MJ, Perumal NB, Tepper RS, Nutt SL, Kaplan MH
Nature immunology 2010 Jun;11(6):527-34

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then intracellular stained with anti-IFN-γ (4S.B3) FITC and MH9A4 PE

Conjugate

Yellow dye