Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF209 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-9 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse IL-9 and recombinant rat IL-9 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P15248
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis.
Tsicopoulos A, Shimbara A, de Nadai P, Aldewachi O, Lamblin C, Lassalle P, Walls AF, Sénéchal S, Levitt RC, Darras J, Hamid Q, Wallaert B
The Journal of allergy and clinical immunology 2004 Mar;113(3):462-9
The Journal of allergy and clinical immunology 2004 Mar;113(3):462-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-9 and Neutralization by Human IL-9 Antibody. Recombinant Human IL-9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF209). The ND50 is typically 2-5 µg/mL.