PA5-111758

antibody from Invitrogen Antibodies

Targeting: TRPV4 CMT2C, OTRPC4, TRP12, VR-OAC, VRL-2, VROAC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Recommended by provider)

Antibody Data

Product number

PA5-111758

Provider

Invitrogen Antibodies

Product name

TRPV4 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TRPV4 was performed in rat brain lysate. Lane 1: TRPV4 (extracellular) Antibody (Product # PA5-111758) at a dilution of 1:200. Lane 2: TRPV4 (extracellular) Antibody preincubated with the negative control antigen.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TRPV4 was performed in rat brain lysate. Lane 1: TRPV4 (extracellular) Antibody (Product # PA5-111758) at a dilution of 1:200. Lane 2: TRPV4 (extracellular) Antibody preincubated with the negative control antigen.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TRPV4 in live intact human U-87 cells. A) Extracellular staining of cells with TRPV4 (extracellular) Antibody (Product # PA5-111758), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B) Merged images of live cells with A.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TRPV4 in live intact Expression of TRPV4 in human U-87 cells using TRPV4 (extracellular) Antibody (Product # PA5-111758), (1:25), (red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. DAPI was used as the counterstaining (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TRPV4 in frozen rat DRG tissue sections using TRPV4 (extracellular) Antibody (Product # PA5-111758). A) TRPV4 labeling appears in neuronal cell bodies. B) Nuclear staining using DAPI as counter stain. C) merged images of A and B.