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LearnACC-124-200UL
antibody from Invitrogen Antibodies
Targeting: TRPV4
CMT2C, OTRPC4, TRP12, VR-OAC, VRL-2, VROAC
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ACC-124-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPV4 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysate: - 1. Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:200). 2. Anti-TRPV4 (extracellular) Antibody , preincubated with TRPV4 (extracellular) Blocking Peptide (#BLP-CC124).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysate: - 1. Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:200). 2. Anti-TRPV4 (extracellular) Antibody , preincubated with TRPV4 (extracellular) Blocking Peptide (#BLP-CC124).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPV4 inhuman U-87 cells - Cell surface detection of TRPV4 in live intact human brain glioblastoma U-87 cells. Extracellular staining of cells using Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:25), (red) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . DAPI was used as the counterstaining (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPV4 inhuman U-87 cells - Cell surface detection of TRPV4 in live intact human brain glioblastoma U-87 cells. Extracellular staining of cells using Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:25), (red) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . DAPI was used as the counterstaining (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPV4 in human U-87 cells - Cell surface detection of TRPV4 in live intact human brain glioblastoma U-87 cells. A. Extracellular staining of cells with Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:25) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. Merged images of live cells with A.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPV4 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 (extracellular) Antibody (# ACC-124). A. TRPV4 labeling appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain. C. merged images of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection ofTRPV4by indirect flow cytometry in live intact human THP-1monocyticleukemia cells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-TRPV4 (extracellular) Antibody (#ACC-124), (2.5μg) + goat- Anti-rabbit-FITC.
