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- References [15]
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- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 16951-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16951-1-AP, RRID:AB_2130861
- Product name
- KLHL3 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated KLHL3 antibody (Cat. #16951-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Insights into the diverse mechanisms and effects of variant CUL3-induced familial hyperkalemic hypertension.
A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.
Combined Kelch-like 3 and Cullin 3 Degradation is a Central Mechanism in Familial Hyperkalemic Hypertension in Mice.
UBR5 is a novel regulator of WNK1 stability.
COP9 signalosome deletion promotes renal injury and distal convoluted tubule remodeling.
KLHL3 deficiency in mice ameliorates obesity, insulin resistance, and nonalcoholic fatty liver disease by regulating energy expenditure.
Renal COP9 Signalosome Deficiency Alters CUL3-KLHL3-WNK Signaling Pathway.
Decreased KLHL3 expression is involved in the pathogenesis of pseudohypoaldosteronism type II caused by cullin 3 mutation in vivo.
cMyBP-C was decreased via KLHL3-mediated proteasomal degradation in congenital heart diseases.
KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3.
Kelch-Like Protein 2 Mediates Angiotensin II-With No Lysine 3 Signaling in the Regulation of Vascular Tonus.
Involvement of selective autophagy mediated by p62/SQSTM1 in KLHL3-dependent WNK4 degradation.
Impaired degradation of WNK by Akt and PKA phosphorylation of KLHL3.
Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice.
KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron.
Sharma P, Chatrathi HE
Cell communication and signaling : CCS 2023 Oct 16;21(1):286
Cell communication and signaling : CCS 2023 Oct 16;21(1):286
A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.
Qi X, Yan Q, Shang Y, Zhao R, Ding X, Gao SJ, Li W, Lu C
Cell death and differentiation 2022 Nov;29(11):2233-2246
Cell death and differentiation 2022 Nov;29(11):2233-2246
Combined Kelch-like 3 and Cullin 3 Degradation is a Central Mechanism in Familial Hyperkalemic Hypertension in Mice.
Maeoka Y, Ferdaus MZ, Cornelius RJ, Sharma A, Su XT, Miller LN, Robertson JA, Gurley SB, Yang CL, Ellison DH, McCormick JA
Journal of the American Society of Nephrology : JASN 2022 Mar;33(3):584-600
Journal of the American Society of Nephrology : JASN 2022 Mar;33(3):584-600
UBR5 is a novel regulator of WNK1 stability.
Jung JU, B Ghosh A, Earnest S, Deaton SL, Cobb MH
American journal of physiology. Cell physiology 2022 Jun 1;322(6):C1176-C1186
American journal of physiology. Cell physiology 2022 Jun 1;322(6):C1176-C1186
COP9 signalosome deletion promotes renal injury and distal convoluted tubule remodeling.
Cornelius RJ, Nelson JW, Su XT, Yang CL, Ellison DH
American journal of physiology. Renal physiology 2022 Jul 1;323(1):F4-F19
American journal of physiology. Renal physiology 2022 Jul 1;323(1):F4-F19
KLHL3 deficiency in mice ameliorates obesity, insulin resistance, and nonalcoholic fatty liver disease by regulating energy expenditure.
Jang JH, Lee JW, Cho MJ, Hwang B, Kwon MG, Kim DH, Lee NK, Lee J, Park YJ, Yang YR, Kim J, Kim YH, An TH, Oh KJ, Bae KH, Park JG, Min JK
Experimental & molecular medicine 2022 Aug;54(8):1250-1261
Experimental & molecular medicine 2022 Aug;54(8):1250-1261
Renal COP9 Signalosome Deficiency Alters CUL3-KLHL3-WNK Signaling Pathway.
Cornelius RJ, Si J, Cuevas CA, Nelson JW, Gratreak BDK, Pardi R, Yang CL, Ellison DH
Journal of the American Society of Nephrology : JASN 2018 Nov;29(11):2627-2640
Journal of the American Society of Nephrology : JASN 2018 Nov;29(11):2627-2640
Decreased KLHL3 expression is involved in the pathogenesis of pseudohypoaldosteronism type II caused by cullin 3 mutation in vivo.
Yoshida S, Araki Y, Mori T, Sasaki E, Kasagi Y, Isobe K, Susa K, Inoue Y, Bomont P, Okado T, Rai T, Uchida S, Sohara E
Clinical and experimental nephrology 2018 Dec;22(6):1251-1257
Clinical and experimental nephrology 2018 Dec;22(6):1251-1257
cMyBP-C was decreased via KLHL3-mediated proteasomal degradation in congenital heart diseases.
Wang L, Lai G, Chu G, Liang X, Zhao Y
Experimental cell research 2017 Jun 1;355(1):18-25
Experimental cell research 2017 Jun 1;355(1):18-25
KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3.
Sasaki E, Susa K, Mori T, Isobe K, Araki Y, Inoue Y, Yoshizaki Y, Ando F, Mori Y, Mandai S, Zeniya M, Takahashi D, Nomura N, Rai T, Uchida S, Sohara E
Molecular and cellular biology 2017 Apr 1;37(7)
Molecular and cellular biology 2017 Apr 1;37(7)
Kelch-Like Protein 2 Mediates Angiotensin II-With No Lysine 3 Signaling in the Regulation of Vascular Tonus.
Zeniya M, Morimoto N, Takahashi D, Mori Y, Mori T, Ando F, Araki Y, Yoshizaki Y, Inoue Y, Isobe K, Nomura N, Oi K, Nishida H, Sasaki S, Sohara E, Rai T, Uchida S
Journal of the American Society of Nephrology : JASN 2015 Sep;26(9):2129-38
Journal of the American Society of Nephrology : JASN 2015 Sep;26(9):2129-38
Involvement of selective autophagy mediated by p62/SQSTM1 in KLHL3-dependent WNK4 degradation.
Mori Y, Mori T, Wakabayashi M, Yoshizaki Y, Zeniya M, Sohara E, Rai T, Uchida S
The Biochemical journal 2015 Nov 15;472(1):33-41
The Biochemical journal 2015 Nov 15;472(1):33-41
Impaired degradation of WNK by Akt and PKA phosphorylation of KLHL3.
Yoshizaki Y, Mori Y, Tsuzaki Y, Mori T, Nomura N, Wakabayashi M, Takahashi D, Zeniya M, Kikuchi E, Araki Y, Ando F, Isobe K, Nishida H, Ohta A, Susa K, Inoue Y, Chiga M, Rai T, Sasaki S, Uchida S, Sohara E
Biochemical and biophysical research communications 2015 Nov 13;467(2):229-34
Biochemical and biophysical research communications 2015 Nov 13;467(2):229-34
Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice.
Susa K, Sohara E, Rai T, Zeniya M, Mori Y, Mori T, Chiga M, Nomura N, Nishida H, Takahashi D, Isobe K, Inoue Y, Takeishi K, Takeda N, Sasaki S, Uchida S
Human molecular genetics 2014 Oct 1;23(19):5052-60
Human molecular genetics 2014 Oct 1;23(19):5052-60
KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron.
Louis-Dit-Picard H, Barc J, Trujillano D, Miserey-Lenkei S, Bouatia-Naji N, Pylypenko O, Beaurain G, Bonnefond A, Sand O, Simian C, Vidal-Petiot E, Soukaseum C, Mandet C, Broux F, Chabre O, Delahousse M, Esnault V, Fiquet B, Houillier P, Bagnis CI, Koenig J, Konrad M, Landais P, Mourani C, Niaudet P, Probst V, Thauvin C, Unwin RJ, Soroka SD, Ehret G, Ossowski S, Caulfield M, International Consortium for Blood Pressure (ICBP), Bruneval P, Estivill X, Froguel P, Hadchouel J, Schott JJ, Jeunemaitre X
Nature genetics 2012 Mar 11;44(4):456-60, S1-3
Nature genetics 2012 Mar 11;44(4):456-60, S1-3
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 16951-1-AP(KLHL3 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The KLHL3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human KLHL3. This antibody recognizes human, mouse, rat antigen. The KLHL3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
