Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 16951-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16951-1-AP, RRID:AB_2130861
- Product name
- KLHL3 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated KLHL3 antibody (Cat. #16951-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Renal COP9 Signalosome Deficiency Alters CUL3-KLHL3-WNK Signaling Pathway.
Decreased KLHL3 expression is involved in the pathogenesis of pseudohypoaldosteronism type II caused by cullin 3 mutation in vivo.
cMyBP-C was decreased via KLHL3-mediated proteasomal degradation in congenital heart diseases.
KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3.
Kelch-Like Protein 2 Mediates Angiotensin II-With No Lysine 3 Signaling in the Regulation of Vascular Tonus.
Involvement of selective autophagy mediated by p62/SQSTM1 in KLHL3-dependent WNK4 degradation.
Impaired degradation of WNK by Akt and PKA phosphorylation of KLHL3.
Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice.
KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron.
Cornelius RJ, Si J, Cuevas CA, Nelson JW, Gratreak BDK, Pardi R, Yang CL, Ellison DH
Journal of the American Society of Nephrology : JASN 2018 Nov;29(11):2627-2640
Journal of the American Society of Nephrology : JASN 2018 Nov;29(11):2627-2640
Decreased KLHL3 expression is involved in the pathogenesis of pseudohypoaldosteronism type II caused by cullin 3 mutation in vivo.
Yoshida S, Araki Y, Mori T, Sasaki E, Kasagi Y, Isobe K, Susa K, Inoue Y, Bomont P, Okado T, Rai T, Uchida S, Sohara E
Clinical and experimental nephrology 2018 Dec;22(6):1251-1257
Clinical and experimental nephrology 2018 Dec;22(6):1251-1257
cMyBP-C was decreased via KLHL3-mediated proteasomal degradation in congenital heart diseases.
Wang L, Lai G, Chu G, Liang X, Zhao Y
Experimental cell research 2017 Jun 1;355(1):18-25
Experimental cell research 2017 Jun 1;355(1):18-25
KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3.
Sasaki E, Susa K, Mori T, Isobe K, Araki Y, Inoue Y, Yoshizaki Y, Ando F, Mori Y, Mandai S, Zeniya M, Takahashi D, Nomura N, Rai T, Uchida S, Sohara E
Molecular and cellular biology 2017 Apr 1;37(7)
Molecular and cellular biology 2017 Apr 1;37(7)
Kelch-Like Protein 2 Mediates Angiotensin II-With No Lysine 3 Signaling in the Regulation of Vascular Tonus.
Zeniya M, Morimoto N, Takahashi D, Mori Y, Mori T, Ando F, Araki Y, Yoshizaki Y, Inoue Y, Isobe K, Nomura N, Oi K, Nishida H, Sasaki S, Sohara E, Rai T, Uchida S
Journal of the American Society of Nephrology : JASN 2015 Sep;26(9):2129-38
Journal of the American Society of Nephrology : JASN 2015 Sep;26(9):2129-38
Involvement of selective autophagy mediated by p62/SQSTM1 in KLHL3-dependent WNK4 degradation.
Mori Y, Mori T, Wakabayashi M, Yoshizaki Y, Zeniya M, Sohara E, Rai T, Uchida S
The Biochemical journal 2015 Nov 15;472(1):33-41
The Biochemical journal 2015 Nov 15;472(1):33-41
Impaired degradation of WNK by Akt and PKA phosphorylation of KLHL3.
Yoshizaki Y, Mori Y, Tsuzaki Y, Mori T, Nomura N, Wakabayashi M, Takahashi D, Zeniya M, Kikuchi E, Araki Y, Ando F, Isobe K, Nishida H, Ohta A, Susa K, Inoue Y, Chiga M, Rai T, Sasaki S, Uchida S, Sohara E
Biochemical and biophysical research communications 2015 Nov 13;467(2):229-34
Biochemical and biophysical research communications 2015 Nov 13;467(2):229-34
Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice.
Susa K, Sohara E, Rai T, Zeniya M, Mori Y, Mori T, Chiga M, Nomura N, Nishida H, Takahashi D, Isobe K, Inoue Y, Takeishi K, Takeda N, Sasaki S, Uchida S
Human molecular genetics 2014 Oct 1;23(19):5052-60
Human molecular genetics 2014 Oct 1;23(19):5052-60
KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron.
Louis-Dit-Picard H, Barc J, Trujillano D, Miserey-Lenkei S, Bouatia-Naji N, Pylypenko O, Beaurain G, Bonnefond A, Sand O, Simian C, Vidal-Petiot E, Soukaseum C, Mandet C, Broux F, Chabre O, Delahousse M, Esnault V, Fiquet B, Houillier P, Bagnis CI, Koenig J, Konrad M, Landais P, Mourani C, Niaudet P, Probst V, Thauvin C, Unwin RJ, Soroka SD, Ehret G, Ossowski S, Caulfield M, International Consortium for Blood Pressure (ICBP)., Bruneval P, Estivill X, Froguel P, Hadchouel J, Schott JJ, Jeunemaitre X
Nature genetics 2012 Mar 11;44(4):456-60, S1-3
Nature genetics 2012 Mar 11;44(4):456-60, S1-3
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 16951-1-AP(KLHL3 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The KLHL3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human KLHL3. This antibody recognizes human, mouse, rat antigen. The KLHL3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.