PA5-49930

antibody from Invitrogen Antibodies

Targeting: LAMA5

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-49930

Provider

Invitrogen Antibodies

Product name

Laminin alpha-5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The antibody detects endogenous levels of total Laminin alpha-5 protein.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Spatiotemporal extracellular matrix modeling for in situ cell niche studies.

Olesen K, Rodin S, Mak WC, Felldin U, Österholm C, Tilevik A, Grinnemo KH
Stem cells (Dayton, Ohio) 2021 Dec;39(12):1751-1765

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

6 FIGURE Region of interest identified by the StEMM is also present in the native heart and partly defined by laminins. The region of interest which was identified in the StEMM was confirmed by immunohistochemistry of native rat hearts: (A) CD146 expression was clustered in the middle of the AVJ region (dotted region), as well as in the PVN (dashed circle). B, PDGFR-beta was also expressed in the PVN, in the aortic valves and aortic root, but not in the AVJ. C, LAMA4 was more generally expressed in the heart, with predominance in the AVJ. D, LAMA5 was also found in all parts of the heart, but with no predominant regions. E, POSTN was identified in the aortic valves and root with thin streaks into the AVJ. F, The aortic valves and root together with the PVN also stained positive for COL1, but not the AVJ. In the figure: scale bars = 200 mum. AVJ, atrioventricular junction; COL1, collagen 1; LAMA4, laminin alpha4; LAMA5, laminin alpha5; PDGFR, platelet derived growth factor receptor; POSTN, periostin; PVN, perivascular niche; StEMM, spatiotemporal extracellular matrix model